SubtiBank SubtiBank
ytcA
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ytcA

similar to to UDP-sugar dehydrogenase
locus
BSU_30860
pI
5.94
mw
46.67 kDa
protein length
428 aa Sequence Blast
gene length
1287 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    3,155,725 3,157,011

    The protein

    Catalyzed reaction/ biological activity

  • H2O + 2 NAD+ + UDP-α-D-glucose --> 3 H+ + 2 NADH + UDP-α-D-glucuronate (according to UniProt)

    • Protein family

  • UDP-glucose/GDP-mannose dehydrogenase family (with [protein|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|TuaD] and [protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|Ugd], according to UniProt)

    • Paralogous protein(s)

  • [protein|DF8CD0B3997A7A4CF4821A7D64E9898422FD17E0|TuaD], [protein|45AFD434F5262BFBE4ED6DF3D18DAED61FF317D1|Ugd]

    • Structure

  • [PDB|4A7P] (from ''Sphingomonas elodea'', 39% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|59024C05AA0106719C81E45E3B060A6F710B2690|ytcA]-[gene|A51B5F9F86D2F7B690712B9EC181B1C07B312E02|ytcB]-[gene|6911DFB27F6A23F3775DAC5C289EFA163FBD1E56|ytcC]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|22383849,15699190,12480901], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

    • regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: repression, [Pubmed|26577401], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

    • regulation

  • expressed late during sporulation in the mother cell ([protein|6FAD8804AA32B84819DDE57C0AF63F208FB9FFD1|SigKC], [SW|GerE]) [Pubmed|26577401,15699190,12480901]
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    Biological materials

    Mutant

  • MGNA-A280 (ytcA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/280 NBRP B. subtilis, Japan]
  • BKE30860 ([gene|59024C05AA0106719C81E45E3B060A6F710B2690|ytcA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACCTGAGCTCCTTTAC, downstream forward: _UP4_ATCTGTACGGGAGTTGGCCG
  • BKK30860 ([gene|59024C05AA0106719C81E45E3B060A6F710B2690|ytcA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30860 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACCTGAGCTCCTTTAC, downstream forward: _UP4_ATCTGTACGGGAGTTGGCCG

    • References

  • 22383849,26577401