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yesT [2019-06-24 10:41:47]
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yesT [2019-06-24 10:41:47]

rhamnogalacturonan acetylesterase
locus
BSU07020
pI
5.51
mw
25.80 kDa
protein length
232 aa Sequence Blast
gene length
699 bp Sequence Blast
function
pectin utilization
product
rhamnogalacturonan esterase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.26|Utilization of pectin]

    • Gene

    Coordinates
    768,137 768,835

    The protein

    Protein family

  • 'GDSL' lipolytic enzyme family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|YxiM], [protein|578B79EB64B804FCAACA73AB22458481371A815C|YesY]

    • Structure

  • [PDB|2O14] ([protein|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|YxiM], 29% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|BF0E202739BFD461E418655ECD9C3425AC556FA3|rhgR]-[gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]-[gene|E4C4AEBA8DAEF7DA1B49432213B2B3EA2AF6B34B|yesU]-[gene|8FE909876A7A21B436CA0CFB90ED190BAD17C8E1|yesV]-[gene|65704140C3B95619D6C0962CB40452D373E2740F|yesW]-[gene|217BAA94FB926CDA7CA8800BC6B08371792CEE31|yesX]-[gene|578B79EB64B804FCAACA73AB22458481371A815C|yesY]-[gene|3226901F1D7C1F21296AB974CAE3656C18BE8F69|yesZ]
    description
    [Pubmed|19651770]

    regulatory mechanism

  • [protein|BF0E202739BFD461E418655ECD9C3425AC556FA3|RhgR]: activation, [Pubmed|19651770], in [regulon|BF0E202739BFD461E418655ECD9C3425AC556FA3|RhgR regulon]

    • regulation

  • induced by pectin ([protein|search|RhgR]) [Pubmed|19651770]
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    Biological materials

    Mutant

  • MGNA-B451 (yesT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1450 NBRP B. subtilis, Japan]
  • BKE07020 ([gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCTTCATCACATGTTCTC, downstream forward: _UP4_CTTGTGAGCCGGGAGGGAAA
  • BKK07020 ([gene|585CA3154C0405697C4707E5B1D9359963999C54|yesT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCTTCATCACATGTTCTC, downstream forward: _UP4_CTTGTGAGCCGGGAGGGAAA

    • References

  • 19651770,17957779,17449691