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mdh [Wed Aug 24 2016 13:14:45 GMT+0200 (CEST)]
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mdh [Wed Aug 24 2016 13:14:45 GMT+0200 (CEST)]

malate dehydrogenase
locus
BSU29120
pI
4.00
mw
33.00 kDa
function
TCA cycle
product
malate dehydrogenase
essential
no
ec
1.1.1.37
synonyms
citH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|membrane proteins], [SW|phosphoproteins], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|CcpC regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,978,734 -> 2,979,672

    The protein

    Catalyzed reaction/ biological activity

  • (S)-malate NAD = oxaloacetate NADH (according to Swiss-Prot)

    • Protein family

  • MDH type 3 family (according to Swiss-Prot)

    • Modification

  • phosphorylated on Arg-156 [Pubmed|22517742]
  • phosphorylation on Ser-149 [Pubmed|17218307]

    • [SW|Cofactors]

  • NAD

    • Effectors of protein activity

  • Inhibited by Mg2, Ca2, Zn2, Ag2 and Hg2 [Pubmed|14284712] [Pubmed|922015]
  • Inhibited by oxaloacetate (above 1mM) and malate (above 7,7mM) [Pubmed|14284712]

    • Structure

  • [PDB|1EMD] (''E.coli'')

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot), membrane associated [Pubmed|18763711]

    • [SW|Interactions]

  • [protein|search|CitZ]-[protein|search|Mdh]-[protein|search|Icd] (this is the core of the TCA cycle metabolon) [Pubmed|20933603]
  • [protein|search|Mdh]-[protein|search|PckA] (this interaction occurs under gluconeogenic, but not under glycolytic conditions) [Pubmed|20933603]
  • [protein|search|Mdh]-[protein|search|Ndh] [Pubmed|20933603]
  • [protein|search|Mdh]-[protein|search|CitB] [Pubmed|20933603]
  • [protein|search|Mdh]-[protein|search|CitG] [Pubmed|20933603]
  • [protein|search|Mdh]-[protein|search|SucC] [Pubmed|20933603]
  • [protein|search|Mdh]-[protein|search|Icd] [Pubmed|24325460]

    • Additional information

  • The enzyme is a tetramer [Pubmed|14284712]
  • extensive information on the structure and enzymatic properties of Mdh can be found at [http://www.proteopedia.org/wiki/index.php/Malate_dehydrogenase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]'' [Pubmed|8045899]
  • ''[protein|search|icd]-[protein|search|mdh]'' [Pubmed|8045899]

    • [SW|Sigma factor]

  • ''[protein|search|citZ]'': [protein|search|SigA] [Pubmed|8045899]
  • ''[protein|search|icd]'': [protein|search|SigA] [Pubmed|8045899]

    • Regulation

  • ''[protein|search|citZ]'': catabolite repression ([protein|search|CcpA]) [Pubmed|12100558]
  • ''[protein|search|citZ]'': repression by glucose glutamate ([protein|search|CcpC]) [Pubmed|12100558]
  • ''[protein|search|mdh]'': constitutive

    • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression, [protein|search|CcpC]: transcription repression [Pubmed|12100558]
  • [protein|search|CcpC]: transcription repression (molecular inducer: citrate) [Pubmed|10656796]

    • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP719(spc) & GP1150(spc), available in [SW|Jörg Stülke]'s lab
  • GP790 (''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]''::''kan''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP385: for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab
  • pGP1123 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP380]) (available in [SW|Jörg Stülke]'s lab)
  • pGP1755 (expression / purification of Mdh-S149A, with N-terminal His-tag from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP1764 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab)
  • GP1438(''mdh''-''Strep'' ''(spc)'') & GP1440(''mdh''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • GP2331 (''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]''::''kan''), Cre-recombinase is integrated in ''[protein|search|sacA]'',available in [SW|Jörg Stülke]'s lab
  • GP2333 (''[protein|search|citZ]-[protein|search|icd]-[protein|search|mdh]''::''lox72''), Cre-recombinase is integrated in ''[protein|search|sacA]'', available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1431 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1130 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • **

    • References

  • 10656796,18763711,14284712,922015,12100558,17218307,8550482,8045899,20933603,22517742,23136871,24325460,15378759,24571712