glycine betaine ABC transporter (binding protein)
function
compatible solute transport
product
glycine betaine ABC transporter (binding protein)
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.4|Uptake of compatible solutes for osmoprotection][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][SW|Categories] containing this gene/protein
[SW|ABC transporters], [SW|coping with hyper-osmotic stress], [SW|membrane proteins]This gene is a member of the following [SW|regulons]
[SW|RemA regulon]Gene
Coordinates on the chromosome (coding sequence)
323,119 -> 324,000
The protein
Catalyzed reaction/ biological activity
binding for subsequent uptake of glycine betaine [Pubmed|24561588]binding for subsequent uptake of dimethylglycine [Pubmed|24561588]Structure
[PDB|2B4L] (complex with glycine betaine), [PDB|3CHG] (in complex with DMSA)[SW|Localization]
associated to the membrane (via [SW|OpuAB]) [Pubmed|10092453][SW|Interactions]
[SW|OpuAA]-[SW|OpuAB]-[SW|OpuAC] [Pubmed|10092453]Expression and Regulation
Operon
''[SW|opuAA]-[SW|opuAB]-[SW|opuAC]'' [Pubmed|22383849][SW|Sigma factor]
[protein|search|SigA] [Pubmed|23175650]Regulation
induced by osmotic stress [Pubmed|23175650]repressed by intracellular glycine betaine [Pubmed|23175650]Regulatory mechanism
[protein|search|RemA]: transcription activation [Pubmed|23646920]Biological materials
Expression vector
pGP2926 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s labLabs working on this gene/protein
[SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]References
10092453,9335265,16645306,16225868,16445940,7622480,18567662,21296969,22383849,23175650,23646920,24561588,25012968 
