SubtiBank SubtiBank
opuAC [2019-02-19 19:14:02]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

opuAC [2019-02-19 19:14:02]

glycine betaine and arsenobetaine [SW|ABC transporter] (binding protein)
locus
BSU03000
pI
8.02
mw
32.06 kDa
protein length
293 aa Sequence Blast
gene length
882 bp Sequence Blast
function
compatible solute transport
product
glycine betaine and arsenobetaine [SW|ABC transporter] (binding protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.4|Uptake of compatible solutes for osmoprotection]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    323,119 324,000

    The protein

    Catalyzed reaction/ biological activity

  • binding for subsequent uptake of glycine betaine and arsenobetaine [pubmed|29159878,24561588]
  • binding for subsequent uptake of dimethylglycine [Pubmed|24561588]

    • Structure

  • [PDB|2B4L] (complex with glycine betaine)
  • [PDB|3CHG] (in complex with DMSA)
  • [PDB|5NXX] (in complex with arsenobetaine) [pubmed|29159878]

    • [SW|Localization]

  • associated to the membrane (via [protein|5F989C57010ACFC03E37AF5A894153F432520921|OpuAB]) [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|FB2220730013A98D7C979062A4A451518B92DF09|opuAA]-[gene|5F989C57010ACFC03E37AF5A894153F432520921|opuAB]-[gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|23175650], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]

    • regulation

  • induced by osmotic stress [Pubmed|23175650]
    • view in new tab

    Biological materials

    Mutant

  • BKE03000 ([gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCCTATGATTTTTTTAA, downstream forward: _UP4_TAATCAAAAAAGCAGCCTGT
  • BKK03000 ([gene|546407EFD8128D1F74443991B8D600F668FBD7A1|opuAC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GATGCCTATGATTTTTTTAA, downstream forward: _UP4_TAATCAAAAAAGCAGCCTGT

    • Expression vectors

  • pGP2926 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jrg Stlke]'s lab

    • labs

  • [SW|Erhard Bremer], University of Marburg, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi homepage]

    • References

    Reviews

  • 27935846

    • Original publications

  • 10092453,9335265,16645306,16225868,16445940,7622480,18567662,21296969,22383849,23175650,23646920,24561588,25012968,29159878