SubtiBank SubtiBank
yoaI
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yoaI

similar to oxygenase component of 4-hydroxyphenylacetate 3-monooxygenase
locus
BSU_18620
pI
5.13
mw
43.01 kDa
protein length
483 aa Sequence Blast
gene length
1452 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]
  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    2,031,439 2,032,890

    The protein

    Catalyzed reaction/ biological activity

  • 4-hydroxyphenylacetate + FADH2 + O2 --> 3,4-dihydroxyphenylacetate + FAD + H+ + H2O (according to UniProt)

    • Protein family

  • FADH(2)-utilizing monooxygenase family (single member, according to UniProt)

    • [SW|Cofactors]

  • FAD (according to UniProt)

    • Structure

  • [PDB|2YYG] (from ''Thermus thermophilus'', 42% identity) [Pubmed|17804419]

    • Expression and Regulation

    Operons

    genes
    [gene|5224B7AD3B669120DBCC071540F6B37D11FD5260|yoaI]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A838 (yoaI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/838 NBRP B. subtilis, Japan]
  • BKE18620 ([gene|5224B7AD3B669120DBCC071540F6B37D11FD5260|yoaI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAAGTTTCGCTCCTTC, downstream forward: _UP4_TGAAAAAACAGCGGGAAAAG
  • BKK18620 ([gene|5224B7AD3B669120DBCC071540F6B37D11FD5260|yoaI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTAAGTTTCGCTCCTTC, downstream forward: _UP4_TGAAAAAACAGCGGGAAAAG

    • References

  • 17804419