glutamine-fructose-6-phosphate transaminase
function
cell wall synthesis
product
glutamine-fructose-6-phosphate transaminase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][SW|Categories] containing this gene/protein
[SW|cell wall synthesis], [SW|biosynthesis of cell wall components], [SW|essential genes]This gene is a member of the following [SW|regulons]
[SW|glmS ribozyme]Gene
Coordinates on the chromosome (coding sequence)
200,277 -> 202,079
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
L-glutamine D-fructose 6-phosphate = L-glutamate D-glucosamine 6-phosphate (according to Swiss-Prot)Structure
[PDB|2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) [Pubmed|18295797]the ribozyme: [PDB|3G8S], [PDB|3G9C], [PDB|3G8T], [PDB|3G95], [PDB|3G96] (all for the ribozyme from ''Bacillus anthracis''), [PDB|2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')[SW|Localization]
cytoplasm (according to Swiss-Prot)Additional information
:subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]Expression and Regulation
Operon
''[protein|search|cdaA]-[protein|search|cdaR]-[protein|search|glmM]-[protein|search|glmS]'' [Pubmed|23192352][SW|Sigma factor]
[protein|search|SigA] [Pubmed|22211522]Regulation
repressed by glucosamine, N-acetylglucosamine, N-propionylglucosamine or N-formylglucosamine [Pubmed|14343123]''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([protein|search|glmS] [SW|ribozyme])Regulatory mechanism
''glmS'' [SW|ribozyme]: glucosamine 6-phosphate binds the leader mRNA, and a [SW|riboswitch] with [SW|ribozyme] activity cleaves off the ''[protein|search|glmS]'' section from the mRNA, resulting in stopp of transcript elongationAdditional information
subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]A [protein|search|ncRNA] is predicted between '[protein|search|glmM]' and '[protein|search|glmS]' [PubMed|20525796]Labs working on this gene/protein
[SW|Wade Winkler], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]References
Reviews
18279655,24769284,12859215,20709015 The ''glmS'' Ribozyme
18079181,16484375,16784238,15096624,16990543,17114942,16484375,15029187,17283212,16298301,19228039,21317896,21395279,24318896 Other Original Publications
14343123,17981983,11160890,18295797,20525796,23192352,23876412,22211522 
