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glmS
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glmS

glutamine-fructose-6-phosphate transaminase
locus
BSU_01780
pI
4.80
mw
65.16 kDa
protein length
600 aa Sequence Blast
gene length
1803 bp Sequence Blast
function
cell wall synthesis
product
glutamine-fructose-6-phosphate transaminase
essential
yes
ec
2.6.1.16
synonyms
gcaA,ybxD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    200,277 202,079

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • D-fructose 6-phosphate + L-glutamine --> D-glucosamine 6-phosphate + L-glutamate (according to UniProt)

    • [SW|Domains]

  • [SW|Glutamine amidotransferase type-2 domain] (aa 2-217) (according to UniProt)
  • [SW|SIS domain] 1 (aa 283-422) (according to UniProt)
  • [SW|SIS domain] 2 (aa 452-590) (according to UniProt)

    • Effectors of protein activity

  • activity is stimulated by interaction with [protein|7150BABA5086D5E2EB8102E4901A216F43576282|GlmR] [pubmed|32994436,30248093]

    • Structure

  • [PDB|2VF4] (GlmS from ''E. coli'', 39% identity, 58% similarity) [Pubmed|18295797]
  • the ribozyme: [PDB|3G8S], [PDB|3G9C], [PDB|3G8T], [PDB|3G95], [PDB|3G96] (all for the ribozyme from ''Bacillus anthracis''), [PDB|2HO7] (the ribozyme from ''Thermonanaerobacter tengcongensis'')

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

    • Expression and Regulation

    Operons

    genes
    [gene|AFE2A9998219162FE32E95628B3CA9071099B61A|cdaA]-[gene|FB23F2AB0E2A82658009D115E5876E7AF1BDE5FD|cdaR]-[gene|F022ACB2CBB8DA46B392CE1D26474093645F26DA|glmM]-[gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS]
    description
    [Pubmed|23192352]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|22211522], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
    • view in new tab

    additional information

  • ''glmS'' is only expressed in the absence of glucosamine 6-phosphate ([SW|GlmS ribozyme])
  • glucosamine 6-phosphate binds the leader mRNA, and a [SW|riboswitch] with [SW|ribozyme] activity cleaves off the [gene|4E23D2043D51C587027ABBF6335F148295E90B8F|glmS] section from the mRNA, resulting in stopp of transcript elongation

    • labs

  • [SW|Wade Winkler], University of Texas, USA, [http://www.utsouthwestern.edu/findfac/professional/0,,68018,00.html Homepage]

    • References

    Reviews

  • 18279655,24769284,12859215,20709015,12044898

    • The ''glmS'' Ribozyme

  • 18079181,16484375,16784238,15096624,16990543,17114942,16484375,15029187,17283212,16298301,19228039,21317896,21395279,24318896,28192411

    • Other Original Publications

  • 14343123,17981983,11160890,18295797,20525796,23192352,23876412,22211522,30105781,30248093,32994436