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helD
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helD

DNA 3-5 helicase IV, stimulates [SW|transcription] in an ATP-dependent manner by enhancing transcriptional cycling and elongation
locus
BSU_33450
pI
6.08
mw
89.74 kDa
protein length
774 aa Sequence Blast
gene length
2325 bp Sequence Blast
function
[SW|transcription], [SW|DNA repair/ recombination]
product
DNA 3-5 helicase IV
essential
no
ec
3.6.4.12
synonyms
yvgS
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    3,434,327 3,436,651

    The protein

    Catalyzed reaction/ biological activity

  • ATP + H2O --> ADP + H+ + phosphate (according to UniProt)

    • Protein family

  • [SW|helicase family] (according to UniProt)

    • [SW|Domains]

  • [SW|UvrD-like helicase ATP-binding domain] (aa 212-610) (according to UniProt)

    • Structure

  • [PDB|3DMN] (C-terminal domain of the protein from ''Lactobacillus plantarum'', 39% identity)

    • [SW|Localization]

  • cytoplasm (according to UniProt)

    • additional information

  • [protein|4DE90422754DA1FD9EFE58766EACB99CE6171207|HelD] may interact with [SW|RNA polymerase] [pubmed|21710567]

    • Expression and Regulation

    Operons

    genes
    [gene|4DE90422754DA1FD9EFE58766EACB99CE6171207|helD]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-A456 (yvgS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/456 NBRP B. subtilis, Japan]
  • 1A899 ( ''helD''::''erm''), available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A899&Search=1A899 BGSC]
  • BKE33450 ([gene|4DE90422754DA1FD9EFE58766EACB99CE6171207|helD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33450 BGSC] as well as in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs, [Pubmed|28189581], upstream reverse: _UP1_CATGAACGGCACCTCCAGAA, downstream forward: _UP4_TGAAGAGAAGGGAGTCCTTC
  • BKK33450 ([gene|4DE90422754DA1FD9EFE58766EACB99CE6171207|helD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33450 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATGAACGGCACCTCCAGAA, downstream forward: _UP4_TGAAGAGAAGGGAGTCCTTC

    • References

  • 24520113,21710567,30186259,30972737