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tlpB [2019-11-11 12:56:58]
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tlpB [2019-11-11 12:56:58]

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein
locus
BSU_31230
pI
4.73
mw
71.36 kDa
protein length
662 aa Sequence Blast
gene length
1989 bp Sequence Blast
function
control of chemotaxis
product
methyl-accepting chemotaxis protein
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.4|Membrane-bound chemoreceptors]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    3,204,067 3,206,055

    The protein

    Paralogous protein(s)

  • [protein|8333CD46F704F03A22482CAA98DEFCC945362A10|McpC], [protein|E4E1020B799A1B403BDB4847B91B02D64D161AF3|McpB], [protein|F02E3080214A327E131ECDE0DBC52C57EFDFE2CE|McpA], [protein|1DE26CDEE952142C1303C822F0E2A63AE09F721B|TlpA]
  • [SW|Domains]

  • [SW|Cache domain] (aa 153-228) (according to UniProt)
  • [SW|HAMP domain] (aa 203-355) (according to UniProt)
  • [SW|Methyl-accepting transducer domain] (aa 374-610) (according to UniProt)
  • [SW|Localization]

  • cell membrane [Pubmed|21515776]
  • Expression and Regulation

    Operons

    genes
    [gene|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB]
    description
    [Pubmed|8188684]

    sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|8188684], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]
  • additional information

  • in minimal medium, TlpB is present with 2,500 +/- 740 molecules per cell [PubMed|21515776]
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    Biological materials

    Mutant

  • BKE31230 ([gene|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCGTTGACTCCTCCT, downstream forward: _UP4_TAATGAAACGAGAAAGCGGC
  • BKK31230 ([gene|4CD44AD8FE68516C84889EAA2137828E06B8A30B|tlpB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTCTCGTTGACTCCTCCT, downstream forward: _UP4_TAATGAAACGAGAAAGCGGC
  • References

  • 8188684,21515776