glutamine phosphoribosyldiphosphate amidotransferase
function
purine biosynthesis
product
glutamine phosphoribosyldiphosphate amidotransferase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides][SW|Categories] containing this gene/protein
[SW|biosynthesis/ acquisition of nucleotides]This gene is a member of the following [SW|regulons]
[SW|G-box], [SW|PurR regulon]Gene
Coordinates on the chromosome (coding sequence)
705,441 -> 706,871
The protein
Catalyzed reaction/ biological activity
5-phospho-beta-D-ribosylamine diphosphate L-glutamate = L-glutamine 5-phospho-alpha-D-ribose 1-diphosphate H2O (according to Swiss-Prot)Structure
[PDB|1GPH], [PDB|1AO0] (complex with ADP and GMP)Additional information
:subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]Expression and Regulation
Operon
''[protein|search|purE]-[protein|search|purK]-[protein|search|purB]-[protein|search|purC]-[protein|search|purS]-[protein|search|purQ]-[protein|search|purL]-[protein|search|purF]-[protein|search|purM]-[protein|search|purN]-[protein|search|purH]-[protein|search|purD]'' [Pubmed|3036807][SW|Sigma factor]
[protein|search|SigA] [Pubmed|3036807]Regulation
repressed in the presence of purine nucleotides ([protein|search|PurR]) [Pubmed|7638212]repressed in the presence of adenine or adenosine([protein|search|PurR]) [Pubmed|2536750]repressed in the presence of guanine([protein|search|G-box]) [Pubmed|3036807]Regulatory mechanism
[PurR]]: transcription repression [Pubmed|7638212][protein|search|PurR]: transcription repression (molecular inducer: PRPP) [Pubmed|2536750][protein|search|G-box]: transcription termination/ antitermination ([SW|riboswitch]) [http://www.ncbi.nlm.nih.gov/sites/entrez/3036807 PubMed1] [http://www.ncbi.nlm.nih.gov/sites/entrez/12787499 PubMed2]Additional information
subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids= 17981983 PubMed]References
Reviews
Original publications
6315725,12923093,6794614,2553706,6401710,6771260,2495277,109433,6814966,6411716,6411717,6794613,3036807,8197456,7638212,25023436 
