ATP-dependent deoxyribonuclease (subunit A), required for efficient survival and replication restart after replication-transcription conflicts
function
DNA repair/ recombination
product
ATP-dependent deoxyribonuclease (subunit A))
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence][SW|Categories] containing this gene/protein
[SW|DNA repair/ recombination], [SW|genetic competence]This gene is a member of the following [SW|regulons]
[SW|ComK regulon]Gene
Phenotypes of a mutant
''[protein|search|recO] [protein|search|addA]-[protein|search|addB]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]The protein
Catalyzed reaction/ biological activity
the enzyme is functional as a heterodimer of the [protein|search|AddA] and [protein|search|AddB] subunits, that it is a rapid and processive DNA helicase, and that it catalyses DNA unwinding using one single-stranded DNA motor of 3'→5' polarity located in the [protein|search|AddA] subunit [Pubmed|21071401]the [protein|search|AddB] subunit contains a second putative ATP-binding pocket, but this does not contribute to the observed helicase activity and may instead be involved in the recognition of recombination hotspot sequences [Pubmed|21071401]Protein family
uvrD-like helicase C-terminal domain (according to Swiss-Prot)Structure
[PDB|3U4Q] (the [protein|search|AddA]-[protein|search|AddB]-DNA complex) [Pubmed|22307084][SW|Interactions]
[protein|search|AddA]-[protein|search|AddB] [Pubmed|10756102,21071401]Expression and Regulation
Operon
''[protein|search|addB]-[protein|search|addA]-[protein|search|sbcD]-[protein|search|sbcC]-[protein|search|hlpB]'' [Pubmed|22383849][SW|Sigma factor]
[protein|search|SigA] [Pubmed|7746142]Regulation
positive control by [protein|search|ComK] [Pubmed|7746142]Biological materials
Mutant
GP1106 ([protein|search|addA]-[protein|search|addB], spc), available in [SW|Jörg Stülke]'s labLabs working on this gene/protein
[SW|Mark Dillingham], Bristol, U.K. ([http://www.bris.ac.uk/biochemistry/research/md.html homepage])References
Reviews
23202527,20116346,22933559,19542287,25486468 Original publications
8387145,15610857,7746142,19129187,15009890,10756102,9781875,16632468,17570399,20350930,21809208,21821766,22307084,24682829,24670664,22383849,21071401,8752329,8752323,25939832,26001966,8510642 
