SubtiBank SubtiBank
addA [2019-09-05 09:36:50]
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addA [2019-09-05 09:36:50]

ATP-dependent deoxyribonuclease (subunit A), required for efficient survival and replication restart after replication-transcription conflicts
locus
BSU_10630
pI
5.13
mw
140.85 kDa
protein length
1232 aa Sequence Blast
gene length
3699 bp Sequence Blast
function
DNA repair/ recombination
product
ATP-dependent deoxyribonuclease (subunit A))
essential
no
synonyms
recE5
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]

    • Gene

    Coordinates
    1,139,807 1,143,505

    Phenotypes of a mutant

  • ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]'' double mutants are extremely sensitive against DNA damaging agents [Pubmed|26001966]

    • The protein

    Catalyzed reaction/ biological activity

  • the enzyme is functional as a heterodimer of the [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|AddA] and [protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|AddB] subunits, that it is a rapid and processive DNA helicase, and that it catalyses DNA unwinding using one single-stranded DNA motor of 3'5' polarity located in the [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|AddA] subunit [Pubmed|21071401]
  • the [protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|AddB] subunit contains a second putative ATP-binding pocket, but this does not contribute to the observed helicase activity and may instead be involved in the recognition of recombination hotspot sequences [Pubmed|21071401]
  • ATP + H2O --> ADP + H+ + phosphate (according to UniProt)

    • Protein family

  • [SW|helicase family] (according to UniProt)

    • [SW|Domains]

  • [SW|UvrD-like helicase ATP-binding domain](aa 9-481) (according to UniProt)
  • [SW|[protein|search|UvrD]-like helicase C-terminal domain] (aa 508-798) (according to UniProt)

    • Structure

  • [PDB|3U4Q] (the [protein|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|AddA]-[protein|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|AddB]-DNA complex) [Pubmed|22307084]

    • Expression and Regulation

    Operons

    genes
    [gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB]-[gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|4D89BFFB1073407A7A1762AFD18B49F3414D6787|sbcD]-[gene|ED825D5B3290BA8F9C92477B1FCC433ADB3C8449|sbcC]-[gene|74E2AB77354355549D14152D3566C9B953A4CB31|hlpB]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7746142], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

    • regulation

  • positive control by [protein|search|ComK] [Pubmed|7746142]
    • view in new tab

    Biological materials

    Mutant

  • GP1106 ([gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]-[gene|55A1EF8A10CB398CCD3FDD06D9483FE0DCE31C64|addB], spc), available in [SW|Jörg Stülke]'s lab
  • BKE10630 ([gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCTGTCCATGTGCTGTCTG, downstream forward: _UP4_TAGCGAGATCCATAAGCTCC
  • BKK10630 ([gene|49E8BFEC1CAB77DD91E0ED0791EBD480E4871239|addA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATCTGTCCATGTGCTGTCTG, downstream forward: _UP4_TAGCGAGATCCATAAGCTCC

    • labs

  • [SW|Mark Dillingham], Bristol, U.K. ([http://www.bris.ac.uk/biochemistry/research/md.html homepage])

    • References

    Reviews

  • 23202527,20116346,22933559,19542287,25486468

    • Original publications

  • 8387145,15610857,7746142,19129187,15009890,10756102,9781875,16632468,17570399,20350930,21809208,21821766,22307084,24682829,24670664,22383849,21071401,8752329,8752323,25939832,26001966,8510642