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qoxA [2019-06-28 12:54:09]
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qoxA [2019-06-28 12:54:09]

cytochrome aa3 quinol oxidase (subunit II)
locus
BSU38170
pI
4.80
mw
36.10 kDa
protein length
321 aa Sequence Blast
gene length
966 bp Sequence Blast
function
respiration
product
cytochrome aa3 quinol oxidase (subunit II)
essential
no
ec
1.10.3.-
synonyms
ipa-37d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.2|Respiration] → [category|SW 2.1.2.1|Terminal oxidases]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,917,296 3,918,261

    Phenotypes of a mutant

  • inactivation of ''[gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]'' restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]

    • The protein

    Catalyzed reaction/ biological activity

  • Ubiquinol-8 + O2 = Ubiquinone-8 + H2O (according to Swiss-Prot)

    • Protein family

  • cytochrome c oxidase subunit 2 family (according to Swiss-Prot)

    • Structure

  • [PDB|1FFT] ([protein|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|QoxA]-[protein|CBF2DCFFD8195304F942ECBA8596444F0AC47CC6|QoxB]-[protein|CD4520EF427AEFDCF028AE063BC373FA6DCE379E|QoxC] complex from ''E. coli'', 35% identity) [Pubmed|11017202]

    • [SW|Localization]

  • cell membrane [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]-[gene|CBF2DCFFD8195304F942ECBA8596444F0AC47CC6|qoxB]-[gene|CD4520EF427AEFDCF028AE063BC373FA6DCE379E|qoxC]-[gene|03335DC50CED7B38E2380640AE108ADA09A2CBDF|qoxD]
    description
    [Pubmed|1316894]

    regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • [protein|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|CitB]: mRNA binding, in [regulon|E15E9F88B0E03FE8834C646369D2F4B4E713EF41|CitB regulon]

    • regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

    • additional information

  • the presence of an iron-responsive element bound by [SW|CitB] between ''[SW|feuA]'' and ''[SW|feuB]'' suggests iron-dependent regulation by [SW|CitB] [Pubmed|10468622]
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    Biological materials

    Mutant

  • BKE38170 ([gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCATCCTTCCTATTGATG, downstream forward: _UP4_TGATATTGATCAGGAAAGGA
  • BKK38170 ([gene|48A9E1E39838BFFCF12CADF0A8D8E6FFDCA6F175|qoxA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38170 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCATCCTTCCTATTGATG, downstream forward: _UP4_TGATATTGATCAGGAAAGGA

    • References

  • 11073895,1316894,20351111,10551842,18763711,24779955,22211522,11017202,27503613