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mgtE [2019-05-08 09:46:52]
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mgtE [2019-05-08 09:46:52]

primary magnesium transporter
locus
BSU13300
pI
4.61
mw
50.68 kDa
protein length
451 aa Sequence Blast
gene length
1356 bp Sequence Blast
function
magnesium uptake
product
primary magnesium transporter
essential
yes
synonyms
ykoK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.5|Metal ion transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,396,013 1,397,368

    Phenotypes of a mutant

  • essential [Pubmed|28189581]
  • a ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]'' mutant has a growth defect on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|CitM]) [Pubmed|24415722]
  • a ''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE] [gene|D281B88EC444F8175AB50FB2CD24B51F4E2C96F8|yfjQ]'' mutant does not grow on rich media, this can be suppressed by the addition of 25 mM Mg2+, moreover the mutant grows well on minimal medium in the presence of citrate (due to the activity of [protein|C0DED68802EF78D2BC16507234AAC3B60D236E68|CitM]) [Pubmed|24415722]

    • The protein

    Protein family

  • SLC41A transporter family (according to Swiss-Prot)

    • [SW|Domains]

  • 2 [SW|CBS domain]s (140-203, 204-260)

    • Structure

  • [PDB|2YVX] (from ''Thermus thermophilus'', 34% identity) [Pubmed|17700703]
  • [PDB|2QBZ] (the mgtE riboswitch)

    • [SW|Localization]

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [regulon|M-box|M-box]: termination/antitermination, Mg-responsive [protein|C245476CE35028167510F67153F392D91447553F|MgtE riboswitch], in [regulon|M-box|M-box]

    • regulation

  • induced at low magnesium concentration ([regulon|M-box|M-box]) [Pubmed|17803910]
    • view in new tab

    additional information

  • expression is increased as a result of loop duplication in [protein|F3DE42C41EF57DC5DFFF0D28129F2CE5DB781D99|L22] [pubmed|30853217]

    • Biological materials

    Mutant

  • MGNA-B313 (mgtE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1312 NBRP B. subtilis, Japan]
  • GP2854 (''Δ''''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]''::''ermC''), available in [SW|Jörg Stülke]'s lab
  • GP2762 (''Δ''''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]''::''spec''), available in [SW|Jörg Stülke]'s lab
  • GP2763 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''kan'' ''Δ''''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]''::''spec''), available in [SW|Jörg Stülke]'s lab

    • Expression vectors

  • pGP2927 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • GP2869 (based on [SW|pAC5]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP2768 ''mgtE-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 24079267

    • Original publications

  • 17803910,15096624,21315082,22970223,24415722,25182490,17700703,25367295,28189581,19798051,17671366,28747715,18832160,29967120,30853217