SubtiBank SubtiBank
yrkA [2019-09-06 08:24:20]
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yrkA [2019-09-06 08:24:20]

similar to magnesium exporter
locus
BSU_26610
pI
4.92
mw
48.82 kDa
protein length
434 aa Sequence Blast
gene length
1305 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,718,959 2,720,263

    The protein

    Protein family

  • [SW|UPF0053 family](according to UniProt)

    • Paralogous protein(s)

  • [protein|A459410312A926365B45CEB4694DE399B38820A2|YugS], [protein|B2E9D026B668C8530C406353E3400059388566FB|YhdT], [protein|BE0777DE7C40825D4DB7252EA84AAB3892578529|MpfA], [protein|E53B8D437B848B637AEC8C1BB9428EC8B6EB280E|YqhB]

    • [SW|Domains]

  • [SW|DUF21 domain] (5-201)
  • 2 [SW|CBS domain]s (aa 220-282, aa 289-346) (according to UniProt)

    • Structure

  • [PDB|4HG0] (CorC from E. coli, corresponds to the [SW|CBS domain]s and the C-terminal transporter-associated domain)

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • BKE26610 ([gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE26610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGATTTGCCTTACCTAG, downstream forward: _UP4_TAACATCTGGATAAAACACA
  • BKK26610 ([gene|45F03AB13292BBF0554785C7C02FE29033EDD742|yrkA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK26610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGATTTGCCTTACCTAG, downstream forward: _UP4_TAACATCTGGATAAAACACA

    • Expression vectors

  • pGP2932 (N-terminal His-tag, purification from ''E. coli'', in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • References

  • 27933050,31415562