SubtiBank SubtiBank
pdhB
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pdhB

pyruvate dehydrogenase (E1 beta subunit)
locus
BSU_14590
pI
4.55
mw
35.32 kDa
protein length
325 aa Sequence Blast
gene length
978 bp Sequence Blast
function
links glycolysis and TCA cycle
product
pyruvate dehydrogenase (E1 beta subunit)
essential
no
ec
1.2.4.1
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.4|TCA cycle]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    1,529,445 1,530,422

    Phenotypes of a mutant

  • defects in sporulation and unable to grow on glucose as single carbon source [Pubmed|11976308]
  • poor growth [pubmed|28189581]
  • non-transformable [pubmed|28189581]

    • The protein

    Catalyzed reaction/ biological activity

  • [dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-lipoyl-L-lysine + H+ + pyruvate --> [dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-(S8-acetyldihydrolipoyl)-L-lysine + CO2 (according to UniProt)

    • Paralogous protein(s)

  • [protein|5F953C3C91EB65A031D04A1D91F1CF501F8EFD09|AcoB], [protein|A024921961A786294199EA12E04456C890ED8D8C|BkdAB]

    • Kinetic information

  • Michaelis-Menten [Pubmed|6414463]

    • Modification

  • phosphorylation on (Ser-302 OR Ser-306) [Pubmed|17218307]

    • Effectors of protein activity

  • Inhibited thiamine 2-thiothiazolone diphosphate and NADH [Pubmed|6414463]
  • Low sensibility to NADPH

    • Structure

  • [PDB|1W88] (E1 in complex with subunit binding domain of E2, ''Geobacillus stearothermophilus'')

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]-[gene|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]-[gene|2F40086E35FA32136B9A89C530A86D714FE9460C|pdhC]-[gene|E9BBAE86DF3E536A987179CC394B472F6F710498|pdhD]
    description
    [Pubmed|11976308]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|20081037], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, due to presence of guanine at 1 position of the transcript [Pubmed|20081037], in [regulon|stringent response|stringent response]

    • regulation

  • ''[protein|search|pdhA]'': expression activated by glucose (1.9-fold) [Pubmed|12850135]
    • view in new tab

    Biological materials

    Mutant

  • GP459 (spc), available in [SW|Jörg Stülke]'s lab
  • BKE14590 ([gene|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATTGTCATTTGCGCCA, downstream forward: _UP4_TAATCAAACTGCATAATCGA
  • BKK14590 ([gene|458E967052D1093A0F48AE0E6B6CCA0F52EAC44D|pdhB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14590 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AATCATTGTCATTTGCGCCA, downstream forward: _UP4_TAATCAAACTGCATAATCGA

    • lacZ fusion

  • pGP722 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • labs

  • [SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]

    • References

    Reviews

  • 19476487,9655937,2227213,6805383,24798336

    • Original publications

  • 9352926,15378759,12850135,17218307,18763711,6414463,11976308,28189581