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gmuA
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gmuA

glucomannan-specific permease of the [SW|phosphotransferase systems|phosphotransferase system], EIIA of the [category|SW 1.2.2|PTS]
locus
BSU_05820
pI
5.63
mw
12.21 kDa
protein length
110 aa Sequence Blast
gene length
333 bp Sequence Blast
function
glucomannan uptake and phosphorylation
product
glucomannan-specific lichenan-specific [category|SW 1.2.2|PTS], EIIA component
essential
no
synonyms
ydhN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.25|Utilization of glucomannan]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    626,933 627,265

    The protein

    Protein family

  • [category|SW 1.2.2|PTS] permease, lactose family [Pubmed|10627040]

    • Paralogous protein(s)

  • [protein|E8B6C8E722BEE866878CDD816B7828C132F94FCD|LicA]

    • [SW|Domains]

  • [SW|PTS EIIA domain] type-3 (aa 9-107) (according to UniProt)

    • Structure

  • [PDB|3K1S] (IIA(Cel) from ''B. anthracis'', 42% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|00A4A90685CED4AD46829C45718CF26F1D62F5D3|gmuB]-[gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]-[gene|76324E0A6CAA8E1DD0B0C6FAE1CAB402231BF7CC|gmuC]-[gene|D8C81A8768E9C6B47B5E196B54DC2A3A8256E7E6|gmuD]-[gene|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|gmuR]-[gene|EB6DE119E6FB18413DB2C2B62B114FB787164F74|gmuE]-[gene|35225776F6044513D3E77DBBD6A7A8FE976F506A|gmuF]-[gene|289ACC9D13BBFAA5C083EE525ECCC32DB06E075F|gmuG]
    description
    [Pubmed|18177310]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|18177310], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]: repression, [Pubmed|18177310], in [regulon|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|18177310], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: activation, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • induced by cellobiose ([protein|64CA75A26A08C534EC7ADFC4A6256F07F3D4BFB7|GmuR]) [Pubmed|18177310]
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    Biological materials

    Mutant

  • MGNA-C189 (ydhN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2187 NBRP B. subtilis, Japan]
  • BKE05820 ([gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTGATTCACCATTAA, downstream forward: _UP4_ATATAAAAAATCGGGGTGGA
  • BKK05820 ([gene|44014896157718D3A6190DFDBD663C5CB9DA63FC|gmuA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05820 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATTGATTCACCATTAA, downstream forward: _UP4_ATATAAAAAATCGGGGTGGA

    • References

  • 18177310,10627040,18177310,20817675