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pbpI [2018-02-16 18:15:48]
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pbpI [2018-02-16 18:15:48]

[SW|sporulation]-specific penicillin-binding protein 4b
locus
BSU27310
pI
9.01
mw
65.35 kDa
protein length
584 aa Sequence Blast
gene length
1752 bp Sequence Blast
function
unknown
product
penicillin-binding protein 4b
essential
no
synonyms
yrrR,pbpI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,789,686 → 2,791,440

    The protein

    Protein family

  • transpeptidase family (according to Swiss-Prot)

    • Structure

  • [PDB|3OC2] (PBP3 from Pseudomonas aeruginosa, 23% identity) [pubmed|20974151]

    • [SW|Localization]

  • during vegetative growth: evenly distributed along the membrane [pubmed|14731276]

    • Expression and Regulation

    Operons

    genes
    [gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]-[gene|9892E5BFE90EC934A4047795BC4B328656128C09|yrrS]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|14679248,15758244], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|15699190,16497325], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

    • regulation

  • expressed early during sporulation in both cell compartments ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE] [Pubmed|14679248,15758244], [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF] [Pubmed|15699190,16497325])
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    Biological materials

    Mutant

  • BKE27310 (Δ[gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAACACCTCACCC, downstream forward: _UP4_TAGAACAACTTCTCAGCACA
  • BKK27310 (Δ[gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAAAACACCTCACCC, downstream forward: _UP4_TAGAACAACTTCTCAGCACA

    • GFP fusion

  • 2081 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]::pSG1489 (cat Pxyl-gfpa-[gene|42088179E71D4C5D53DC0C089CE5F57540DE4721|pbpI]1-430) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

    • References

  • 14679248,15699190,16497325,15758244,14731276,20974151