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lcfB [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]
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lcfB [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

long-chain fatty-acid-CoA ligase, involved in surfactin production
locus
BSU10270
pI
5.30
mw
56.00 kDa
function
fatty acid degradation
product
long-chain fatty-acid-CoA ligase
essential
no
ec
6.2.1.3
synonyms
yhfL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.3|Utilization of fatty acids]

    • [SW|Categories] containing this gene/protein

  • [SW|utilization of lipids]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpA regulon], [SW|FadR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,100,980 -> 1,102,521

    The protein

    Catalyzed reaction/ biological activity

  • ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis [Pubmed|20797616]

    • Protein family

  • ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|LcfA], [protein|search|YhfT], [protein|search|YngI]

    • Expression and Regulation

    Operon

  • ''lcfB'' [Pubmed|17189250]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|17189250]

    • Regulation

  • induced in the presence of long-chain fatty acids ([protein|search|FadR]) [Pubmed|17189250]
  • subject to carbon catabolite repression ([protein|search|CcpA]-[PtsH|search|HPr(Ser-P)] [Pubmed|21398533]

    • Regulatory mechanism

  • [protein|search|FadR]: transcription repression [Pubmed|17189250]
  • [protein|search|CcpA]-[PtsH|search|HPr(Ser-P)]: transcription repression [Pubmed|21398533]

    • Biological materials

    Mutant

  • available in [SW|Mohamed Marahiel]'s lab [Pubmed|20797616]

    • References

  • 17189250,17919287,20797616