You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

hfq [2019-07-02 09:26:02]

RNA chaperone

locus

BSU17340

8.70

8.38 kDa

protein length

73 aa Sequence Blast

gene length

222 bp Sequence Blast

function

unknown

product

RNA chaperone

essential

synonyms

ymaH

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

Hide small RNAs Zoom:

categories

[category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.2|RNA chaperones]

Gene

Coordinates

1,867,373 1,867,594

The protein

Protein family

hfq family (according to Swiss-Prot)

Structure

[PDB|3HSB] (complex with an RNA aptamer) [Pubmed|22053080]

Expression and Regulation

Operons

genes

[gene|09C9CDA3EA23B751C7135DEDF0FE2563BA69FF1D|miaA]-[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

description

[pubmed|22383849]

view in new tab

genes

description

[pubmed|22383849]

regulation

expressed during [SW|sporulation ][Pubmed|22383849]

view in new tab

genes

[gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]

description

[Pubmed|23457461]

regulation

expression (mRNA levels) is quite constant during growth in minimal medium [Pubmed|23457461]

view in new tab

Biological materials

Mutant

GP22 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::cat), available in [SW|Jörg Stülke]'s lab

BKE17340 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT, downstream forward: _UP4_TAGATCAAAAAATGCCATGT

BKK17340 ([gene|40D3FE1A512703B090316C31099956DB6A7F515B|hfq]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17340 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTCGTCCTCCTTGAT, downstream forward: _UP4_TAGATCAAAAAATGCCATGT

Expression vectors

pGP2166: expression of ''hfq''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

lacZ fusion

pGP460 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

GP1067 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

Reviews

17395525,15009882,21760622,26773797

Original publications

12850135,20445260,23457461,22965117,22053080,24932523,25150227,25915524,27049793,27581927,12093755