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proJ [Tue Oct 25 2016 13:01:32 GMT+0200 (CEST)]
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proJ [Tue Oct 25 2016 13:01:32 GMT+0200 (CEST)]

glutamate 5-kinase
locus
BSU18470
pI
5.00
mw
40.00 kDa
function
osmoadaptive de novo production of proline
product
glutamate 5-kinase
essential
no
ec
2.7.2.11
synonyms
yohA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids], [SW|coping with hyper-osmotic stress]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,015,733 -> 2,016,848

    The protein

    Catalyzed reaction/ biological activity

  • ATP L-glutamate = ADP L-glutamate 5-phosphate (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|ProB]

    • Structure

  • [PDB|4Q1T] (from ''Burkholderia thailandensis'', 39% identity)

    • Expression and Regulation

    Operon

  • ''[protein|search|proH]-[protein|search|proJ]'' [Pubmed|21296969]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|21784929]

    • Regulation

  • expressed under conditions of osmotic stress [Pubmed|21784929]
  • induced by cold stress [Pubmed|21296969]

    • Biological materials

    Mutant

  • GP817 (spc) available in the [SW|Stülke] lab
  • 1A635 ( ''proJ''::''erm''), [Pubmed|3015878], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A635&Search=1A635 BGSC]

    • References

    Reviews

  • 25367752

    • Original publications

  • 21296969,25344233,21784929