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rnjA [Fri Feb 03 2017 13:28:46 GMT+0100 (CET)]
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rnjA [Fri Feb 03 2017 13:28:46 GMT+0100 (CET)]

RNase J1
locus
BSU14530
pI
5.00
mw
61.00 kDa
function
RNA processing
product
RNase J1
essential
no
synonyms
ykqC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.1|Exoribonucleases]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • [SW|Categories] containing this gene/protein

  • [SW|RNAses], [SW|essential genes]

    • Gene

    Coordinates on the chromosome (coding sequence)
    1,523,118 -> 1,524,785

    Phenotypes of a mutant

  • the mutant is strongly impaired in [SW|sporulation], [SW|genetic competence] and many other phenotypes [Pubmed|23504012]
  • it is not possible to construct a ''[SW|rnjA] [SW|rny]'' double mutant [Pubmed|23504012]

    • The protein

    Catalyzed reaction/ biological activity

  • endonuclease and 5'-3' exonuclease
  • degrades [SW|SR5] and ''[SW|gapA]'' RNAs [SW|27449348]

    • Protein family

  • RNase J subfamily (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|RnjB]

    • Effectors of protein activity

  • initeraction with [SW|GapA]-[SW|YkzW] stimulates activity [Pubmed|27449348]

    • Structure

  • [PDB|3ZQ4] [Pubmed|21893285]

    • [SW|Localization]

  • cytoplasm, colocalizes with the ribosomes [Pubmed|27708634]

    • [SW|Interactions]

  • part of the [SW|RNA degradosome] [Pubmed|19193632]
  • [protein|search|RnjA]-[protein|search|RnjB] [Pubmed|19193632,20025672,27708634]
  • [protein|search|RnjA]-[protein|search|PfkA] [Pubmed|19193632]
  • [protein|search|RnjA]-[protein|search|PnpA] [Pubmed|19193632], K(D) of the interaction: 250 nM [Pubmed|22198292]
  • [protein|search|RnjA]-[protein|search|Rny] [Pubmed|19193632,21803996]
  • [protein|search|RnjA]-[protein|search|CshA] [Pubmed|20572937]
  • [SW|GapA]-[SW|RnjA] [Pubmed|27449348], about 1% of all [SW|GapA] molecules participate in this interaction [Pubmed|27449348], this interaction is stabilized in the presence of [SW|YkzW] [Pubmed|27449348]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • required for ''[protein|search|thrS]'' RNA processing, involved in maturation of the 5’-end of the16S rRNA

    • Expression and Regulation

    Operon

  • ''[protein|search|rpoY]-[protein|search|rnjA]'' [Pubmed|24187087]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|24187087]

    • Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
  • translation of [protein|search|YkzG] and [protein|search|RnjA] is coupled, and this coupling is required for efficient expression of [rnjA|search|RNase J1] [PubMed|24187087]

    • Biological materials

    Mutant

  • GP41 (''rnjA'' under control of p(xyl)), available in [SW|Jörg Stülke]'s lab
  • SSB342 (''rnjA'' under pspac), cat, available in [SW|Harald Putzer] lab
  • GP2506 (''[protein|search|rnjA]''::''spc''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • for chromosomal expression of RNase J1-Strep (spc): GP1034, available in [SW|Jörg Stülke]'s lab
  • for chromosomal expression of RNase J1-Strep (cat): GP1042, available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • pGP418 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1694 (in pHJS-105 [Pubmed|26110430]), expression of '' rnjA-sfGFP''::''spc'' under a xylose-inducible promoter in '' B. subtilis'', available in [SW|Jörg Stülke]'s lab [Pubmed|27708634]

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1020 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • GP1075 (aphA3), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Harald Putzer], IBPC Paris, France [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.htm Homepage]
  • [SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
  • [SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]

    • References

    Reviews

  • 20458164,21334965,21893280,23403287,21957024,22568516,24064983,25292357,27750368

    • Original publications

  • 25940620,18079181,19553197,17981983,19458035,15831787,18204464,18713320,19193632,17005971,18445592,17512403,17229210,17576666,19210617,19633085,19638340,19850915,19880604,20025672,20418391,20572937,21803996,21862575,21925382,22198292,22412379,23504012,21893285,21893286,21908660,24187087,26253740,26802042,26940229,27708634,27449348