SubtiBank SubtiBank
yhcW [2018-02-10T13:00:10.000Z]
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yhcW [2018-02-10T13:00:10.000Z]

putative glycerol-3-phosphatase
locus
BSU09240
pI
4.63
mw
24.53 kDa
protein length
220 aa Sequence Blast
gene length
660 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

    • Gene

    Coordinates
    997,724 → 998,386

    The protein

    Catalyzed reaction/ biological activity

  • glycerol-3-phosphate -→ glycerol + inorganic phosphate [Pubmed|22353596]

    • Protein family

  • [SW|HAD superfamily]
  • CbbY/cbbZ/gph/yieH family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|CAFAE305B1771DD89E81F75A6713161393BE99A0|PgcM] (31%)

    • Structure

  • [PDB|4UAR] (from ''Rhodobacter Sphaeroides'', 32% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|3DB9048B54C47E262997A2A5A0556AE607F93EA9|yhcW]-[gene|BDAA216E832B8EB267CED6456A0045254DD0E97D|yhcX]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-A660 (yhcW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/660 NBRP B. subtilis, Japan]
  • BKE09240 (Δ[gene|3DB9048B54C47E262997A2A5A0556AE607F93EA9|yhcW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGGGTACAACTCCTTTA, downstream forward: _UP4_CAAAACTAGAAAGGAGCGAG
  • BKK09240 (Δ[gene|3DB9048B54C47E262997A2A5A0556AE607F93EA9|yhcW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09240 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTGGGTACAACTCCTTTA, downstream forward: _UP4_CAAAACTAGAAAGGAGCGAG

    • References

  • 22353596,22383849