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sigW [Thu Jul 07 2016 18:52:46 GMT+0200 (CEST)]
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sigW [Thu Jul 07 2016 18:52:46 GMT+0200 (CEST)]

RNA polymerase ECF-type sigma factor SigW, required for the adaptation to membrane active agents, activated by alkaline shock and by polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100
locus
BSU01730
pI
8.00
mw
21.00 kDa
function
adaptation to membrane-active compounds
product
RNA polymerase ECF-type sigma factor SigW
essential
no
synonyms
ybbL

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.2|Sigma factors]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.1|Sigma factors]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [SW|Categories] containing this gene/protein

  • [SW|transcription], [SW|sigma factors and their control], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|SigW regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    194,849 -> 195,412

    Phenotypes of a mutant

  • sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin [Pubmed|22178969]
  • Inactivation of ''[protein|search|alsR]'', ''[protein|search|sigD]'' and ''[protein|search|sigW]'' increases competitive fitness of ''Bacillus subtilis'' under non-sporulating conditions [Pubmed|22344650]
  • The protein

    Protein family

  • ECF subfamily (according to Swiss-Prot)
  • Effectors of protein activity

  • [protein|search|RsiW] acts as antagonist of SigW (anti-SigW)
  • [SW|Interactions]

  • [protein|search|RsiW]-[protein|search|SigW] [Pubmed|14993308]
  • [protein|search|SigW]-[protein|search|RpoB]-[protein|search|RpoC]
  • Expression and Regulation

    Operon

  • ''[protein|search|sigW]-[protein|search|rsiW]'' [Pubmed|12207695]
  • [SW|Sigma factor]

  • [protein|search|SigW] [Pubmed|12207695]
  • Regulation

  • induced under conditions of alkali shock or in the presence of the toxin [protein|search|SdpC] ([protein|search|SigW]) [Pubmed|12207695]
  • repressed during logrithmic growth ([protein|search|AbrB]) [Pubmed|12076816]
  • Regulatory mechanism

  • [protein|search|AbrB]: transcription repression [Pubmed|12076816]
  • Biological materials

    Mutant

  • 1A905 ( ''sigW''::''erm''), [Pubmed|11244082], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A905&Search=1A905 BGSC]
  • Labs working on this gene/protein

  • [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
  • [SW|Thomas Wiegert], University of Bayreuth, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/Thomas Wiegert-Dateien/Thomas Wiegert.html Homepage]
  • References

    Reviews

  • 22381678,24921931,26901131,27344142
  • The [SW|SigW regulon]

  • 9683469,16629676,17675383,11866510,9987136,10960106,23934352,23155385
  • Other original publications

  • 11222589,14993308,16816000,17338440,12207695,11454200,14563871,12076816,19820159,21821766,22344650,14993308,23185515,20817771,21542858,22178969,27137497