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ygaF [2019-10-01 13:31:26]

similar to thioredoxin-dependent hydroperoxide peroxidase

locus

BSU_08720

5.86

17.97 kDa

protein length

157 aa Sequence Blast

gene length

474 bp Sequence Blast

function

resistance against oxidative stress

product

unknown

essential

synonyms

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.9|Resistance against oxidative and electrophile stress/ based on similarity]

Gene

Coordinates

943,891 944,364

The protein

Catalyzed reaction/ biological activity

[protein]-dithiol + hydroperoxide --> [protein]-disulfide + alcohol + H₂O (according to UniProt)

Protein family

[SW|peroxiredoxin family] (according to UniProt)

[SW|Domains]

[SW|Thioredoxin domain] (aa 3-156) (according to UniProt)

Structure

[PDB|5ENU] (from Burkholderia ambifaria, 48% identity)

Expression and Regulation

Operons

genes

[gene|3C139835154FA236066CF2FB82A3C32E3A72D65E|ygaF]-[gene|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR]

description

[Pubmed|22383849]

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Biological materials

Mutant

BKE08720 ([gene|3C139835154FA236066CF2FB82A3C32E3A72D65E|ygaF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTTACCTCCGGATG, downstream forward: _UP4_TAAATCTCTATGAGCCTATG

BKK08720 ([gene|3C139835154FA236066CF2FB82A3C32E3A72D65E|ygaF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTACGTTACCTCCGGATG, downstream forward: _UP4_TAAATCTCTATGAGCCTATG

Expression vector

pGP2338: expression of ''ygaF'' [SW|pBQ200] in ''B. subtilis'' suitable, available in [SW|Jörg Stülke]'s lab

References

10644761