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pbpA [2020-05-28 15:25:08]
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pbpA [2020-05-28 15:25:08]

class B penicillin-binding protein PBP 2A, required for [SW|cell wall synthesis] during cell elongation, part of the [protein|search|Rod complex] for lateral [SW|cell wall synthesis] and control of cell diameter
locus
BSU_25000
pI
9.57
mw
79.96 kDa
protein length
716 aa Sequence Blast
gene length
2151 bp Sequence Blast
function
formation of a rod-shaped peptidoglycan cell wall, spore outgrowth
product
penicillin-binding protein PBP 2A
essential
no
synonyms
yqgF
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.7|Penicillin-binding proteins]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,581,771 2,583,921

    The protein

    Catalyzed reaction/ biological activity

  • required for [SW|cell wall synthesis] during cell elongation [Pubmed|12896990]
  • driver of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] dynamics [Pubmed|21636744,21636745]

    • Protein family

  • [SW|transpeptidase family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|883DFF72888D280A102E53CC1D69A8B1C7BE2907|PbpH]

    • Structure

  • [PDB|3VSK] (from Staphylococcus aureus, 45% identity) [pubmed|22846910]

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]
  • during vegetative growth: evenly distributed along the membrane [pubmed|14731276]
  • localization depends on the availability of peptidoglyan precursors (lipid II) [Pubmed|23895585]

    • Expression and Regulation

    Operons

    genes
    [gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]
    description
    [Pubmed|21815947]

    additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
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    Biological materials

    Mutant

  • MGNA-C461 (yqgF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2459 NBRP B. subtilis, Japan]
  • BKE25000 ([gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG
  • BKK25000 ([gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAACATCACCTTTTCTAT, downstream forward: _UP4_TAAAAAAAAACGCTCACATG

    • GFP fusion

  • 3103 [gene|0467447B10EE7C1FDF86022AB6FBDBABFD32A9E5|trpC]2 [gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]::pSG5043 (cat Pxyl-gfpa-[gene|3B4F035535D6504405567E7C44E72902A11F7447|pbpA]1-804) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jeff Errington] lab

    • labs

  • [SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]

    • References

  • 9851991,9139922,12896990,18957862,20487272,21636744,21636745,21815947,23895585,22846910,14731276,31086310