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dat
function
peptidoglycan precursor biosynthesis
product
D-alanine aminotransferase
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan][category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.15|Biosynthesis/acquisition of L- and D-alanine][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]Gene
Coordinates
1,041,994 1,042,842
The protein
Catalyzed reaction/ biological activity
2-oxoglutarate + D-alanine --> D-glutamate + pyruvate (according to UniProt)Protein family
[SW|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)[SW|Cofactors]
PLPStructure
[PDB|1G2W] (E177S Mutant, Geobacillus stearothermophilus, 43% identity)Expression and Regulation
Operons
genes
[gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat]
description
[Pubmed|22383849]
Biological materials
Mutant
MGNA-B489 (yheM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1488 NBRP B. subtilis, Japan]BKE09670 ([gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAGCCCTCCCTTTTC, downstream forward: _UP4_TAAATGAACGAAGAAAAGAGBKK09670 ([gene|38989B6CB025AE110709E2EA2025EFD425328FFA|dat]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAAAGCCCTCCCTTTTC, downstream forward: _UP4_TAAATGAACGAAGAAAAGAGReferences
