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minD
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minD

[SW|cell division] inhibitor (septum placement), part of the Min system (with Z ring placement), required for the disassembly of polar [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|Z rings] after [SW|cell division]
locus
BSU_27990
pI
4.98
mw
29.26 kDa
protein length
268 aa Sequence Blast
gene length
807 bp Sequence Blast
function
septum placement
product
[SW|cell division] inhibitor
essential
no
synonyms
divIVB1
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.1|The Min system]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,857,776 2,858,582

    Phenotypes of a mutant

  • produces minicells [pubmed|32184253]
  • a ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]'' mutant grows poorly, and the cells are filamentous [Pubmed|24097947]

    • The protein

    Catalyzed reaction/ biological activity

  • The Min system prevents minicell formation adjacent to recently completed division sites by promoting the disassembly of the cytokinetic ring, thereby ensuring that cell division occurs only once per cell cycle [Pubmed|20352045]
  • required for oriC placement during spore development [Pubmed|27059541]

    • Protein family

  • ParA family (with [protein|D6D34E68FB3BAAACB533F98290FD363A5B00B2C6|FlhG] and [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|ParA], according to UniProt)

    • Structure

  • [PDB|4V03] (from ''Aquifex aeolicus'', 51% identity) [Pubmed|25500731]

    • [SW|Localization]

  • polar/ septal at the cell membrane [Pubmed|20566861]
  • membrane binding/ polar localization depends on the proton motive force [Pubmed|20566861]

    • Expression and Regulation

    Operons

    genes
    [gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
    description
    [Pubmed|8459776]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, promoter p2, within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, promoter p4, upstream of [protein|8C94C9598A823A8405B3E1FA0124E21D90845B8E|MinC] [Pubmed|8459776], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|26091431], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

    • regulation

  • constitutively expressed [Pubmed|23701187]
    • view in new tab

    genes
    [gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
    description
    [Pubmed|8459776]

    regulation

  • constitutively expressed [Pubmed|23701187]
    • view in new tab

    genes
    [gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

    • regulation

  • constitutively expressed [Pubmed|23701187]
    • view in new tab

    Biological materials

    Mutant

  • BKE27990 ([gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTTATTACGATAGCCTCAC, downstream forward: _UP4_TAATGTGATAGAATCAAAGA
  • BKK27990 ([gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27990 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGTTATTACGATAGCCTCAC, downstream forward: _UP4_TAATGTGATAGAATCAAAGA

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 26286503,19884039,24367361,28697666

    • Original Publications

  • 8459776,19019154,11886553,9808628,1400224,12368265,10411726,15317782,12492861,20352045,11886553,20566861,18179421,21821766,22457634,21926231,24097947,25374563,25500731,22383849,26091431,27059541,28674273,30092000,32184253