trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBCA of the PTS
function
beta-glucoside uptake and phosphorylation, control of LicT activity
product
trigger enzyme: beta-glucoside-specific phosphotransferase system, EIIBCA
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins][category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.18|Utilization of beta-glucosides][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.4|Control of PRD-type regulators][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.1|Trigger enzymes of the PTS that control the activity of PRD-containing transcription factors][category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.3|Phosphorylation on a Cys residue][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]Gene
Coordinates
4,033,778 → 4,035,607
The protein
Catalyzed reaction/ biological activity
Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine (according to Swiss-Prot)Protein family
[protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] permease, sucrose permease (Scr) family [Pubmed|10627040][SW|Localization]
cell membrane [Pubmed|23475962]Expression and Regulation
Operons
genes
[gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]-[gene|8D98212930CF6613D332BF99452B231CB6426E93|bglH]-[gene|5A4CDE1CFE319E4471A15702ED1632C96270528D|yxiE]
description
[Pubmed|7883710]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7559347], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA] binding site overlaps -35 region) [Pubmed|7559347], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon][protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]: anti-termination, via [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]-dependent [SW|RNA switch], lack of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT]-dependent antitermination in the presence of gucose due to the requirement of [protein|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT] to be phosphorylated by [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|HprK], in [regulon|FAC682CCD66056A8CBB9AE5F4BF51C1AADEBBD2C|LicT regulon]regulation
induced by salicin ([protein|search|LicT]) [Pubmed|7883710]additional information
An [ncRNA|search|antisense RNA] is predicted for '[protein|search|bglP]' [PubMed|20525796]view in new tabBiological materials
Mutant
GP475 (erm), available in the [SW|Stülke] labExpression vector
pGP1290 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Stülke] lab,pGP1300 (expression of ''[gene|35E6C81BE481C3E57CF47B781580368C3AC15D83|bglP]'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Stülke] lablacZ fusion
pGP600 (in [protein|search|pAC6]), available in [SW|Stülke] labReferences
16672620,20525796,10627040,7883710,8626332,7559347,22900538,23475962 
