SubtiBank SubtiBank
rimM
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rimM

16S rRNA processing protein, RNase
locus
BSU_16020
pI
5.22
mw
20.06 kDa
protein length
174 aa Sequence Blast
gene length
525 bp Sequence Blast
function
maturation of 16S rRNA
product
16S rRNA processing protein, RNase
essential
no
synonyms
ylqE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.4|RNases] → [category|SW 3.2.4.4|RNases/ Other]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.2|rRNA modification and maturation]

    • Gene

    Coordinates
    1,674,650 1,675,174

    Phenotypes of a mutant

  • defective in 16S rRNA 3' processing by [protein|E0437C86E5F3707FF1DA13C8B98F73130DED8FB4|YqfG] [pubmed|31003868]

    • The protein

    Protein family

  • RimM family (single member, according to UniProt)

    • [SW|Domains]

  • PRC barrel domain (aa 98-171) (according to UniProt)

    • Structure

  • [PDB|3H9N] (from ''Haemophilus influenzae'', 33% identity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|8F559BFC80FE56EA0389A2336F4E9E6908562BCB|ylxM]-[gene|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh]-[gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP]-[gene|67FB5F6FB0B2701BD8E4FDD8DF06B236DE6A2599|ylqC]-[gene|0AB1FB44E8F28944B82B5EF14A9CDCFEE46DB76B|ylqD]-[gene|34B0FA6E7E25D1E5D8A54B70F5562BD0BD05B498|rimM]-[gene|885ECD9FAAC8BC95D32F049825D6B6244C046112|trmD]-[gene|51368512B8322EDBCA92674EC2B97CF26087D8A9|rplS]
    description
    [Pubmed|29794222]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8335643], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulation

  • the mRNA is processed between [gene|EC025F78D0BCBD91162BAAB9EAB7D3837E0A3208|ffh] and [gene|25C4850812DC216C1BEFBF07DCA71CC6820930B0|rpsP] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
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    Biological materials

    Mutant

  • MGNA-B376 (ylqE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1375 NBRP B. subtilis, Japan]
  • BKE16020 ([gene|34B0FA6E7E25D1E5D8A54B70F5562BD0BD05B498|rimM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGATCACCTCTGGCGA, downstream forward: _UP4_GAATGAAAATCGATTTTTTG
  • BKK16020 ([gene|34B0FA6E7E25D1E5D8A54B70F5562BD0BD05B498|rimM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16020 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGATCACCTCTGGCGA, downstream forward: _UP4_GAATGAAAATCGATTTTTTG

    • References

  • 15496525,31003868