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dnaK
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dnaK

class I heat-shock protein (molecular chaperone)
locus
BSU_25470
pI
4.57
mw
65.83 kDa
protein length
611 aa Sequence Blast
gene length
1836 bp Sequence Blast
function
protein quality control
product
class I heat-shock protein (molecular chaperone)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.2|Chaperones/ protein folding]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

    • Gene

    Coordinates
    2,626,112 2,627,947

    Phenotypes of a mutant

  • reduced [SW|protein secretion] [pubmed|32111210]

    • The protein

    Protein family

  • heat shock protein 70 family (single member, according to UniProt)

    • Modification

  • phosphorylated on Tyr-601 by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|PtkA], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|PtpZ] [Pubmed|27148221,17726680]

    • Structure

  • [PDB|2V7Y] (from ''Geobacillus kaustophilus'', 87% identity) [Pubmed|18400763]
  • [PDB|4ANI] ([protein|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|DnaK]-[protein|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|GrpE] complex from ''Geobacillus kaustophilus'', 87% identity) [Pubmed|22544739]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • membrane-proximal (Spotty) [Pubmed|16479537]
  • recruited to the membrane after ethanol stress [Pubmed|22268681]

    • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Expression and Regulation

    Operons

    genes
    [gene|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|hrcA]-[gene|ECAB685E9038DC03FA2CC659112BB07D30DE5C8C|grpE]-[gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]-[gene|D4C75B39B2DAECB9D85DAD66AAD4F923C9AEBEA7|dnaJ]-[gene|70D717806F5174447E01FF33DC8299CB025888CD|yqeT]-[gene|F8DC71B29D9C9BF4CF5124D0F01A71548306B9F9|yqeU]-[gene|6ADA5AF308D96218414EA92DFF4A57BAEAD0554B|yqeV]
    description
    [Pubmed|9023197]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1339421], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA]: repression, [Pubmed|1339421], in [regulon|2E57DFB4162EF0CE759C1FC42C637E1B9E4B2F7E|HrcA regulon]

    • regulation

  • induced by heat shock ([protein|search|HrcA]) [Pubmed|1339421]
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    Biological materials

    Mutant

  • BKE25470 ([gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTGAATAACCTCCTGCT, downstream forward: _UP4_TAAGTTCTTTTTAGTGTCAG
  • BKK25470 ([gene|30E0AAABB803E577D0D9FBAEF9031319CABD3D89|dnaK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTGAATAACCTCCTGCT, downstream forward: _UP4_TAAGTTCTTTTTAGTGTCAG

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • References

  • 1339421,12224648,10383760,9023197,7540247,7592421,16479537,1339421,17726680,22268681,15378759,22544739,18400763,27148221,32111210