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oppA
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oppA

oligopeptide [SW|ABC transporter] (binding protein)
locus
BSU_11430
pI
5.72
mw
61.32 kDa
protein length
545 aa Sequence Blast
gene length
1638 bp Sequence Blast
function
initiation of [SW|sporulation], competence development
product
oligopeptide [SW|ABC transporter] (binding protein)
essential
no
synonyms
spo0KA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.3|Uptake of peptides]
  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.4|Utilization of peptides]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]

    • Gene

    Coordinates
    1,219,849 1,221,486

    The protein

    Catalyzed reaction/ biological activity

  • required for the uptake of quorum sensing peptides such as [protein|4FA7D6E6316A6FC71C10C692D125833263894020|PhrH] [Pubmed|21908671]

    • Protein family

  • [SW|bacterial solute-binding protein 5 family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|853C570CFB9382449A20BE77B44B4FE44972AA59|DppE]

    • Modification

  • phosphorylation on (Tyr-301 OR Tyr-303) AND Thr-470 [Pubmed|17218307]

    • Structure

  • [PDB|1OLA] (from Salmonella enterica, 36% identity) [pubmed|8202710]

    • [SW|Localization]

  • attached to the cell membrane (via [protein|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|OppB]-[protein|DDBCE73B6AF39E5D669475479186C93F287DA9FC|OppC]) [Pubmed|10092453]
  • at the outer side of the cell: extracellular (signal peptide) [Pubmed|18957862], lipid modification as retention signal [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]-[gene|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[gene|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]-[gene|05752B9C4EA7ACD579D65D028B7DF1861F3840CB|oppD]-[gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]
    description
    [Pubmed|1901616]

    regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, [Pubmed|12823818], in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]
  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, no derepression occurs, however, in a [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY] mutant, due to increased repression by [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC] [Pubmed|25966844], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|10383984], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]

    • regulation

  • expressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]

    • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|trpS]' and '[protein|search|oppA]' [PubMed|20525796]
    • view in new tab

    Biological materials

    Mutant

  • BP67 (spc) available in [SW|Fabian Commichau]'s lab
  • 1S118 ( ''oppA''::''spec''), [Pubmed|11267663], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S118&Search=1S118 BGSC]
  • GP2097 (D(''[gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]-[gene|B0A3C7B253FB1DA1D4BC9D1D564905ECE8A65BC1|oppB]-[gene|DDBCE73B6AF39E5D669475479186C93F287DA9FC|oppC]-[gene|05752B9C4EA7ACD579D65D028B7DF1861F3840CB|oppD]-[gene|38AD697B6C9967B8BD7496E31264A21F6D0A9DEE|oppF]'')::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • BKE11430 (''[gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]''::''erm'', available in the BGSC, in [SW|Fabian Commichau]'s, and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
  • BKE11430 ([gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCGCAAACCCCCTAA, downstream forward: _UP4_TAAGGCTACGTCTGAAAATA
  • BKK11430 ([gene|302DFA46D73E18C4663468ED6033C55056744475|oppA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11430 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCGCAAACCCCCTAA, downstream forward: _UP4_TAAGGCTACGTCTGAAAATA

    • References

  • 1901616,10092453,27231947,11267663,9252573,10960106,12823818,10383984,18957862,18763711,17218307,7997159,20525796,23651456,21908671,25755103,25966844,1899858,23199363,8202710,31426791