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acoL [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]
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acoL [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
locus
BSU08090
pI
5.27
mw
48.00 kDa
function
acetoin utilization
product
acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
essential
no
ec
1.8.1.4
synonyms
yfjH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.2|Utilization of acetoin]

    • [SW|Categories] containing this gene/protein

  • [SW|utilization of specific carbon sources], [SW|sporulation proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|AcoR regulon], [SW|CcpA regulon], [SW|SigL regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    882,266 -> 883,642

    The protein

    Catalyzed reaction/ biological activity

  • Protein N(6)-(dihydrolipoyl)lysine + NAD+ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)

    • Protein family

  • class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|LpdV], [protein|search|PdhD]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • [SW|Interactions]

  • ([protein|search|AcoA]-[protein|search|AcoB])-[protein|search|AcoC]-[protein|search|AcoL]

    • Expression and Regulation

    Operon

  • ''[protein|search|acoA]-[protein|search|acoB]-[protein|search|acoC]-[protein|search|acoL]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]

    • [SW|Sigma factor]

  • [protein|search|SigL] [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]

    • Regulation

  • repressed by glucose ([protein|search|CcpA]) [Pubmed|10666464]
  • induced by acetoin ([protein|search|AcoR]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
  • induced under anaerobic conditions ([protein|search|Fnr]) [Pubmed|16428414]

    • Regulatory mechanism

  • [protein|search|CcpA]: transcription repression [Pubmed|10666464]
  • [protein|search|AcoR]: transcription activation (interaction with [protein|search|SigL]-containing [SW|RNA polymerase]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]
  • [protein|search|Fnr]: indirect positive regulation via [protein|search|NarG]-[protein|search|NarH]-[protein|search|NarJ]-[protein|search|NarI] [Pubmed|16428414]

    • Additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
  • the mRNA is substantially stabilized upon depletion of [Rny|search|RNase Y] [PubMed|21815947]

    • Labs working on this gene/protein

  • [SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]

    • References

  • 11274109,10368162,10666464,16428414,12884008,21815947