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spo0A [2017-07-24 12:51:07]
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spo0A [2017-07-24 12:51:07]

phosphorelay regulator, initiation of sporulation, coordinates DNA replication and initiation of sporulation by binding to sites close to the oriC
locus
BSU24220
pI
5.99
mw
29.54 kDa
protein length
267 aa Sequence Blast
gene length
801 bp Sequence Blast
function
initiation of sporulation
product
phosphorelay response regulator
essential
no
synonyms
spo0C, spo0G, spoIIL, sof-1
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.4|The ultimate target]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.4|The ultimate target]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

    • Gene

    Coordinates
    2,518,023 → 2,518,826

    Phenotypes of a mutant

  • inactivation of ''[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]'' restores beta-lactam resistance in a ''[gene|081DF3EE9FA56209D648C7677188C61CE3AA8E41|sigM]'' mutant [Pubmed|22211522]
  • altered cell death pattern in colonies [Pubmed|23012477]

    • The protein

    Catalyzed reaction/ biological activity

  • activation of gene expression at the onset of [SW|sporulation] ([SW|Spo0A regulon])
  • coordination between [SW|DNA replication] and initiation of [SW|sporulation] by binding to sites close to the ''oriC'' (this limits re-initiation of replication) [Pubmed|23301687]
  • required for [SW|biofilm formation] [Pubmed|11886552]
  • required for [SW|sliding] [Pubmed|26152584]

    • Modification

  • receives phosphorylation from [protein|29D91115BB3AC03538D618E54A5A57F92777EFF6|Spo0B], dephosphorylation by [protein|A574974B6F4FF46DC69E03AE021651C67089866F|Spo0E], direct phosphorylation by [protein|A656321846B2E0D1F39B528E2D8B8E620CCD1148|KinC] upon potassium leakage [Pubmed|19114652]

    • Effectors of protein activity

  • the phosphorylation state affects DNA binding activity
  • interaction with [protein|331993A875907C10C77105FD8DDD86D4412CE405|MecA] inhibits the transcriptional activity of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]∼P [Pubmed|21435029]

    • Structure

  • [PDB|1QMP] (with phosphorylated aspartate, ''Geobacillus stearothermophilus''), [PDB|1FC3] (trans-activation domain, ''Geobacillus stearothermophilus''), [PDB|1LQ1] (complex with DNA)

    • Expression and Regulation

    Operons

    genes
    [gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]
    description
    [Pubmed|11254139]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11254139,1569009], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|11254139,1569009], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|1391039], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

    • regulation

  • expression increases when drowth rate declines [Pubmed|21552330]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C448 (spo0A::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2446 NBRP B. subtilis, Japan]
  • BKG9 (''[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]''::''aphA3''), available in [SW|Jörg Stülke]'s lab
  • SS995 (''[gene|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]''::''ery'') [Pubmed|14761993]

    • Labs working on this gene/protein

  • [SW|Tony Wilkinson], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]
  • [SW|Imrich Barak], Slovak Academy of Science, Bratislava, Slovakia [http://imb.savba.sk/~barak/ homepage]
  • [SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]

    • References

    Reviews

  • 19995980,20030732,20157337,11286862,20833318,20955377,22303284,24914187,26458398,28075389

    • The [SW|Spo0A regulon]

  • 14651647,15687200

    • Other original Publications

  • 14679239,1569009,20413551,20404177,19528067,19581368,8730857,2106683,3157992,11254139,12067336,10869437,16385044,9287005,8127878,9287022,8231806,1905258,16452424,2118505,8207022,12176382,9733708,11069677,12730135,2437099,8288522,16166384,9495766,2118512,26165942,1556084,18296515,7768874,3145384,19114652,20689749,1537790,9658000,8509330,14762002,1846779,2981817,9477965,8600030,18840696,1391039,14976210,19207565,11679073,12270811,11112444,18978066,20154131,14712656,17157871,15060025,9685500,10852876,21552330,22303282,22745669,23301687,23335417,23660663,25225273,25341802,26152584,23012477,23170957,23169620,22412392,21326214,21435029,21478340,21622736,21949067,21980382,22211522,23927765,24123822,27208661,27216630,27215790,27891124,28033323