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yokF [2019-09-17 12:08:04]
similar to micrococcal nuclease
Genomic Context
categories
[category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage][category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]Gene
Coordinates
2,279,977 2,280,867
The protein
Paralogous protein(s)
[protein|91E6B4971F22B5A5F964590F8B14D6C73A4C4840|YncB] [SW|Domains]
TNase-like (aa 66-199) (according to UniProt)Structure
[PDB|4I65] (from Staphylococcus aureus, corresponds to aa 71 ... 198 of YncB, 57% identity)Expression and Regulation
Operons
genes
[gene|27F32FB0F96276325ED93710DF77B949658D728D|yokF]-[gene|C4C5A7CCD4B02EC846A40C52574A1092D5A95F68|yokE]
description
[pubmed|22383849]
view in new tabBiological materials
Mutant
BKE21610 ([gene|27F32FB0F96276325ED93710DF77B949658D728D|yokF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCATTTCCCCTTTTA, downstream forward: _UP4_TGTGAACGATGAAAGGAGATBKK21610 ([gene|27F32FB0F96276325ED93710DF77B949658D728D|yokF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAACTTCATTTCCCCTTTTA, downstream forward: _UP4_TGTGAACGATGAAAGGAGAT