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sunT [2019-09-02 13:18:17]
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sunT [2019-09-02 13:18:17]

[protein|1A6D90298D039FFFD977B2534952BA5E32B3530F|sublancin] 168 lantibiotic [SW|ABC transporter]
locus
BSU_21470
pI
8.39
mw
81.38 kDa
protein length
705 aa Sequence Blast
gene length
2118 bp Sequence Blast
function
[protein|1A6D90298D039FFFD977B2534952BA5E32B3530F|sublancin] export and processing
product
[protein|1A6D90298D039FFFD977B2534952BA5E32B3530F|sublancin] 168 lantibiotic [SW|ABC transporter]
essential
no
synonyms
yolH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.2|Export of antibiotic substances]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.2|SP-beta prophage]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,267,346 2,269,463

    The protein

    Protein family

  • [SW|ABC transporter superfamily] (according to UniProt)

    • [SW|Domains]

  • has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]

    • Structure

  • [PDB|4RY2] (from Clostridium thermocellum, 33% identity) [pubmed|26201595]

    • [SW|Localization]

  • membrane [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|1A6D90298D039FFFD977B2534952BA5E32B3530F|sunA]-[gene|260693EE7F5B5CE2A141149D292405E84B7A5FF4|sunT]-[gene|DB5F719C0A2F610301E174F6890DA9E826C76932|bdbA]-[gene|6B2DC9873B7B693D9D0FC0D773740140D0640C9E|yolJ]-[gene|12CE5AE0BD02C989E803480A1CE5A47D6192D751|bdbB]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [Pubmed|15743949], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]
  • [protein|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh]: activation, [Pubmed|20817675,19465659], in [regulon|5872812AB61E92E2944E926915EB7FEE71BFA6D5|Abh regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]: repression, [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]-[protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|27902860,15743949], in [regulon|6740108089F13116F200C15F35C2E7561E990FEB|DnaA regulon]
  • [protein|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb]: activation, [Pubmed|16306698], in [regulon|7098319D8E88FDC2A629A3F4A21507FD7A649385|YvrHb regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675,17720793], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

    • regulation

  • repressed by [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]-[SW|DnaA] [Pubmed|27902860,15743949]
  • expression starts in the stationary phase [pubmed|30808982]
  • expression is heterogeneous in the population, this is mediated by [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB], [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok], and [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A] [pubmed|30808982]

    • additional information

  • the mRNA is very stable (> 15 min) [pubmed|12884008]
  • the amount of the mRNA is substantially decreased upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
    • view in new tab

    Biological materials

    Mutant

  • BKE21470 (''sunT''::''erm trpC2'') available in the Bacillus Genetic Stock Center and in [SW|Jörg Stülke]'s lab [pubmed|28189581]
  • BKE21470 ([gene|260693EE7F5B5CE2A141149D292405E84B7A5FF4|sunT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTCAATCCCCGCTTTA, downstream forward: _UP4_TCGGAAAATAAGGAGTATTC
  • BKK21470 ([gene|260693EE7F5B5CE2A141149D292405E84B7A5FF4|sunT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATTTCAATCCCCGCTTTA, downstream forward: _UP4_TCGGAAAATAAGGAGTATTC

    • References

    Reviews

  • 23106164

    • Original publications

  • 27902860,722542,11872755,10092453,15743949,16306698,21815947,20817675,26201595