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yqeK

symmetrically cleaving Ap₄A hydrolase

locus

BSU_25630

5.97

21.14 kDa

protein length

186 aa Sequence Blast

gene length

561 bp Sequence Blast

function

degradation of Ap₄A

product

Ap₄A hydrolase

essential

synonyms

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.4|Nucleotide metabolism/ other]

Gene

Coordinates

2,643,215 2,643,775

Phenotypes of a mutant

increased levels of Ap₄A [pubmed|32152217]

The protein

Catalyzed reaction/ biological activity

symmetric cleavage of Ap₄A [pubmed|32152217]

[SW|Domains]

[SW|HD domain] (aa 18-132) (according to UniProt)

Structure

[PDB|3CCG] (the protein from ''Clostridium acetobutylicum'', 46% identity, 76% similarity)

Expression and Regulation

Operons

genes

description

[Pubmed|22383849]

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Biological materials

Mutant

BKE25630 ([gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTCACGCATGCAAGTG, downstream forward: _UP4_AGCTGAACAGGAGGAATTTG

BKK25630 ([gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25630 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGCTTCACGCATGCAAGTG, downstream forward: _UP4_AGCTGAACAGGAGGAATTTG

References

15175311,22383849,32152217