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thiX [2018-05-04 14:34:15]
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thiX [2018-05-04 14:34:15]

thiamine ABC transporter (membrane protein)
locus
BSU13210
pI
10.02
mw
27.89 kDa
protein length
254 aa Sequence Blast
gene length
762 bp Sequence Blast
function
thiamine uptake
product
thiamine ABC transporter (membrane protein)
essential
no
synonyms
ykoC
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.3|Class I ECF transporter]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.3|Biosynthesis/ acquisition of thiamine]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,388,070 → 1,388,834

    The protein

    Protein family

  • cbiQ family (according to Swiss-Prot)

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|57A953B5D71F3FD8269EAF2366B8E2C7A8C79483|thiU]-[gene|9AF27E4CE34B5C4036840A27D93B13213E3324DD|thiV]-[gene|EF69891D9CF0CE57367B38FB3C69DDE1B689FCB7|thiW]-[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]
    description
    [Pubmed|16291685]

    regulatory mechanism

  • [protein|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box]: [SW|RNA switch], via [SW|RNA switch], in [regulon|E195900D971AEE4A790D4579BE5E5A15B81010B8|Thi-box regulon]

    • regulation

  • repressed by thiamine ([protein|search|Thi-box]) [Pubmed|12376536]
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    Biological materials

    Mutant

  • MGNA-B311 (ykoC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1310 NBRP B. subtilis, Japan]
  • BKE13210 (Δ[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AACCGCCGCCGCTTTCACAG, downstream forward: _UP4_TAAAAAATCCGCTATCACAG
  • BKK13210 (Δ[gene|236095EDE02EC9EA7768D9D30CED2345D3B7A1A9|thiX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AACCGCCGCCGCTTTCACAG, downstream forward: _UP4_TAAAAAATCCGCTATCACAG

    • References

  • 10092453,16291685,12376536,18763711