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gcaD [2019-07-19 10:38:05]
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gcaD [2019-07-19 10:38:05]

bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase
locus
BSU_00500
pI
5.65
mw
49.29 kDa
protein length
456 aa Sequence Blast
gene length
1371 bp Sequence Blast
function
cell wall metabolism
product
bifunctional N-acetylglucosamine-1-phosphate
essential
yes
ec
2.7.7.23
synonyms
tms,tms26,glmU
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    56,352 57,722

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • acetyl-CoA + α-D-glucosamine 1-phosphate --> CoA + H+ + N-acetyl-α-D-glucosamine 1-phosphate (according to UniProt)
  • H+ + N-acetyl-α-D-glucosamine 1-phosphate + UTP --> diphosphate + UDP-N-acetyl-α-D-glucosamine

    • Protein family

  • N-terminal domain: N-acetylglucosamine-1-phosphate uridyltransferase family (single member, according to UniProt)
  • C-terminal domain: [SW|Transferase hexapeptide repeat family] (according to UniProt)

    • Structure

  • [PDB|1HM9] (from ''Streptococcus pneumoniae'', 49% identity) [Pubmed|11118459]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|23540C0D84F55135E39A09F48E48AB6F7C270B2E|gcaD]-[gene|37664AAEBFFDE1F7D17EA7C032805EA051FBE6AF|prs]-[gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|8522540], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]
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    Biological materials

    Expression vectors

  • pGP2596: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

    • References

  • 1328164,8522540,11118459,21303765