bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase
function
cell wall metabolism
product
bifunctional N-acetylglucosamine-1-phosphate
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan][category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][SW|Categories] containing this gene/protein
[SW|cell wall synthesis], [SW|biosynthesis of cell wall components], [SW|essential genes]This gene is a member of the following [SW|regulons]
[SW|stringent response]Gene
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate (according to Swiss-Prot)Structure
[PDB|1HM9] (from ''Streptococcus pneumoniae'', 49% identity) [Pubmed|11118459][SW|Localization]
cytoplasm (according to Swiss-Prot)Expression and Regulation
Operon
''[protein|search|gcaD]-[protein|search|prs]-[protein|search|ctc]'' [Pubmed|8522540][SW|Sigma factor]
[protein|search|SigA] [Pubmed|8522540]Regulation
[protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]Biological materials
Expression vector
pGP2596: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s labReferences
1328164,8522540,11118459,21303765 
