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mreD
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mreD

MreD is a [SW|cell shape] determining protein, it couples the cytosolic [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] and [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB]-like proteins to the extracellular peptidoglycan-synthesizing machinery, part of the [protein|search|Rod complex] for lateral [SW|cell wall synthesis] and control of cell diameter
locus
BSU_28010
pI
7.91
mw
19.67 kDa
protein length
172 aa Sequence Blast
gene length
519 bp Sequence Blast
function
[SW|cell shape] determation
product
[SW|cell shape]-determining protein
essential
yes
synonyms
rodB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,859,317 2,859,835

    Phenotypes of a mutant

  • the phenotype of ''mreD'' is similar to that of ''[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]
  • ''mreD'' is essential under normal conditions [Pubmed|12682299]
  • Depletion of MreD leads to a progressive increase in the width and a decrease in the length of the cell and cells become lytic. In the depletion strain, lysis can be prevented and cell growth, but not cell shape, can be recovered by inculaion of Magnesium in the media. This shape defect is consistent with a role for ''mreD'' in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation.

    • The protein

    Catalyzed reaction/ biological activity

  • None/ structural

    • Protein family

  • mreD family (single member, according to UniProt)

    • Structure

  • None

    • [SW|Localization]

  • trans-membrane protein [Pubmed|21636744]
  • during logarithmic growth, [protein|231C30963AFB863424C087CB722446D319976D65|MreD] forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]
  • forms transverse bands as cells enter the stationary phase [Pubmed|21636744]
  • reports on helical structures formed by MreD [Pubmed|20566861] seem to be misinterpretation of data [Pubmed|21636744]

    • Expression and Regulation

    Operons

    genes
    [gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
    description
    [Pubmed|8459776]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, promoter p2, within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, promoter p4, upstream of [protein|8C94C9598A823A8405B3E1FA0124E21D90845B8E|MinC] [Pubmed|8459776], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|26091431], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

    • regulation

  • constitutively expressed [Pubmed|23701187]
    • view in new tab

    genes
    [gene|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|maf]-[gene|2BD11D71AA6D9EFAD8A209C8813CC8B059180C84|radC]-[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]-[gene|6A14D0C7FD94A838BAB3B74AA100EA678F1E4E34|mreC]-[gene|231C30963AFB863424C087CB722446D319976D65|mreD]-[gene|8C94C9598A823A8405B3E1FA0124E21D90845B8E|minC]-[gene|37DAD42A391E2FC506225EAF91B8F21629A401DF|minD]
    description
    [Pubmed|22383849]

    sigma factors

  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, promoter p2 within [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|18179421], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, promoter p1, upstream of [protein|49D996C9AED44A820A214C3DF60AB1B6E1508DEA|Maf] [Pubmed|26091431], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|11948146,11918817,21564336], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

    • regulation

  • constitutively expressed [Pubmed|23701187]
    • view in new tab

    Biological materials

    Mutant

  • A conditional mutant with an inframe deletion of ''mreD'' complemented by a xylose inducible copy at an ectopic locus (strain named 4352) is avaliable from the Errington Group.

    • GFP fusion

  • A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3416 [Pubmed|16101995].

    • labs

  • [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

    • References

    Localization

  • 20566861,21636744,21636745

    • Other original publications

  • 1400224,18156271,16101995,19643765,8459776,18179421,21630458,23879732,22383849,26091431,412835,31086310