SubtiBank SubtiBank
yngE
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yngE

methylcrotonoyl-CoA carboxylase, subunit
locus
BSU_18210
pI
8.36
mw
66.13 kDa
protein length
511 aa Sequence Blast
gene length
1536 bp Sequence Blast
function
mother cell metabolism, leucine utilization
product
methylcrotonoyl-CoA carboxylase, subunit
essential
no
ec
6.4.1.4
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.9|Utilization of branched-chain amino acids]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    1,949,682 1,951,217

    The protein

    Catalyzed reaction/ biological activity

  • 3-methylbut-2-enoyl-CoA + Biotin-CO2 -- 3-methyl-glutaconyl-CoA + biotin [Pubmed|19935659]

    • Protein family

  • AccD/PCCB family (with [protein|E4997526CC534E38413030E90F7A135ECF65DF40|AccD] and [protein|B6A05AD90A4AA553A8CCD8FFF504F5AD204ABAFB|YqjD], according to UniProt)

    • Paralogous protein(s)

  • [protein|B6A05AD90A4AA553A8CCD8FFF504F5AD204ABAFB|YqjD]

    • [SW|Domains]

  • CoA carboxyltransferase N-terminal domain (aa 2-254) (according to UniProt)
  • CoA carboxyltransferase C-terminal domain (aa 260-506) (according to UniProt)

    • Structure

  • [PDB|1VRG] (from Thermotoga maritima, 40% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|BA28DFBAF88A4994B0541D1FF2187312FDE1FA15|yngJ]-[gene|5F73ACDEAFC7F1EB26D55195100C3283FC1F587C|yngI]-[gene|CAF58B4BAEB03353CF80A2862590888D07B48E2C|yngH]-[gene|1FBF1512C28BCE04E7D51247D94DC88C72FBFF07|yngHB]-[gene|EEEE69A98DFAA3327FBC04F1422E959D8B44AF20|yngG]-[gene|D5CCFDE9312909B7E5B98876794569357791C76B|yngF]-[gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]
    description
    [Pubmed|12662922]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|15699190,12662922], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

    • regulation

  • expressed during sporulation in the mother cell ([protein|search|SigE]) [Pubmed|15699190,12662922]
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    Biological materials

    Mutant

  • MGNA-B081 (yngE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1080 NBRP B. subtilis, Japan]
  • BKE18210 ([gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE18210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATAATCCATCAGCATTGCCT, downstream forward: _UP4_TAAAAGCAAGAACTTAGGAG
  • BKK18210 ([gene|2192474E13EAF6D2CF97CB957864FC89ED0118E0|yngE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK18210 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATAATCCATCAGCATTGCCT, downstream forward: _UP4_TAAAAGCAAGAACTTAGGAG

    • References

  • 15699190,12662922,19935659