SubtiBank SubtiBank
rapF [2018-10-15 12:48:04]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

rapF [2018-10-15 12:48:04]

response regulator aspartate phosphatase, anti-activator protein, controls ComA activity
locus
BSU37460
pI
5.18
mw
45.45 kDa
protein length
381 aa Sequence Blast
gene length
1143 bp Sequence Blast
function
control of ComA activity
product
response regulator aspartate phosphatase
essential
no
synonyms
ywhJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.2|Response regulator aspartate phosphatase]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]

    • Gene

    Coordinates
    3,846,001 → 3,847,146

    The protein

    Catalyzed reaction/ biological activity

  • control of [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA] activity [Pubmed|15968044]

    • Protein family

  • RAP family (according to Swiss-Prot)

    • Effectors of protein activity

  • binding of [protein|AEBEDF43C56A9A54716D781D062067B69818FAF4|PhrF] to [protein|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|RapF] results in a conformational change of [protein|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|RapF] and prevents it from binding [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA] [Pubmed|15968044,22215984]

    • Structure

  • [PDB|3ULQ] (structure of the complex between the DNA-binding C-terminal domain of [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA] with [protein|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|RapF]) [Pubmed|22215984]

    • Expression and Regulation

    Operons

    genes
    [gene|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|rapF]-[gene|AEBEDF43C56A9A54716D781D062067B69818FAF4|phrF]
    description
    [Pubmed|11466295]

    sigma factors

  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|11466295], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA]: activation, [Pubmed|15968044], in [regulon|7832489F11F606BCF637EC23BAAB41AD10237EBB|ComA regulon]

    • regulation

  • PhrF accumulates at high cell density
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A035 (rapF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/35 NBRP B. subtilis, Japan]
  • BKE37460 (Δ[gene|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|rapF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTGCAAATCCCTCCC, downstream forward: _UP4_CTTATTCAAGGAGGAGTGAG
  • BKK37460 (Δ[gene|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|rapF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTGCAAATCCCTCCC, downstream forward: _UP4_CTTATTCAAGGAGGAGTGAG

    • References

  • 12850135,15968044,11466295,16091051,22215984