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iolJ
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iolJ

2-deoxy-5-keto-gluconic acid-6-phosphate aldolase, formation of dihydroxyacetone phosphate and malonic semialdehyde (6th reaction)
locus
BSU_39670
pI
4.85
mw
31.22 kDa
protein length
290 aa Sequence Blast
gene length
873 bp Sequence Blast
function
myo-inositol catabolism
product
2-deoxy-5-keto-gluconic acid-6-phosphate aldolase, formation of dihydroxyacetone phosphate and malonic semialdehyde (6th reaction)
essential
no
ec
4.1.2.29
synonyms
fbaB,yxdI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.16|Utilization of inositol]

    • Gene

    Coordinates
    4,073,081 4,073,953

    The protein

    Catalyzed reaction/ biological activity

  • 6-phospho-5-dehydro-2-deoxy-D-gluconate --> 3-oxopropanoate + dihydroxyacetone phosphate (according to UniProt)

    • Protein family

  • class II fructose-bisphosphate aldolase family (with [protein|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|FbaA], according to UniProt)

    • Paralogous protein(s)

  • [protein|800EC1F1CA8C4F2A643EDECBB684347C29CAB0CA|FbaA]

    • Effectors of protein activity

  • inhibited by alpha-keto acids [Pubmed|24624]

    • Structure

  • [PDB|4TO8] (from Staphylococcus aureus, 56% identity) [pubmed|25390935]

    • Expression and Regulation

    Operons

    genes
    [gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]-[gene|0903CCBE053230998A9123F3269868E952C0F76B|iolB]-[gene|AAE60753464EAA32379521531FDCAF64534D5F40|iolC]-[gene|AA79B6F039EC5B79F4419A5F358AE5AE8292E731|iolD]-[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]-[gene|14813DE6C6DF7AB031BA05AED7D31AE9E8F8498E|iolF]-[gene|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG]-[gene|FD64E9D00BB03B510C7F7CA3935558A2195532A9|iolH]-[gene|2742FCE60AA87B2BE5A0B5C34F617E10DDE7F126|iolI]-[gene|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]
    description
    [Pubmed|9226270]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9226270], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR]: repression, [Pubmed|9887260,9226270], in [regulon|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • induced by inositol ([protein|search|IolR]) [Pubmed|9887260,9226270]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B701 (iolJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1700 NBRP B. subtilis, Japan]
  • BKE39670 ([gene|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCACCCCTCCTTTAT, downstream forward: _UP4_TAAAGAGACCAGAAACCGCC
  • BKK39670 ([gene|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39670 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTCACCCCTCCTTTAT, downstream forward: _UP4_TAAAGAGACCAGAAACCGCC

    • labs

  • [SW|Yasutaro Fujita], University of Fukuyama, Japan
  • [[Ken-ichi Yoshida]], Kobe University, Japan

    • References

  • 9226270,18310071,9887260,18310071,25390935