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ssbB [2018-02-10T15:35:46.000Z]
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ssbB [2018-02-10T15:35:46.000Z]

single-strand DNA-binding proteinrequired to internalize and to recombine ssDNA with homologous resident duplex
locus
BSU36310
pI
6.88
mw
12.35 kDa
protein length
113 aa Sequence Blast
gene length
339 bp Sequence Blast
function
required for efficient genetic transformation
product
single-strand DNA-binding protein
essential
no
synonyms
ywpH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]

    • Gene

    Coordinates
    3,740,206 → 3,740,547

    The protein

    Catalyzed reaction/ biological activity

  • binds and protects internalized foreign ssDNA [Pubmed|22373918]
  • RecA-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|DprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|SsbB] as an accessory factor [Pubmed|25138221]

    • Paralogous protein(s)

  • [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|SsbA]

    • Modification

  • phosphorylated on Arg-55 [Pubmed|22517742]
  • phosphorylated on Tyr by [protein|6100EA37592A2C31BC3DE79AD7948A8D6F65F6E1|PtkA], dephosphorylated by [protein|CF73D86DB024575BE8F043A4C3996458F4262E46|PtpZ] [Pubmed|16549871]

    • Effectors of protein activity

  • phosphorylation strongly increases DNA-binding activity [Pubmed|16549871]

    • Structure

  • [PDB|3VDY] (in complex with ssDNA) [Pubmed|22373918]

    • [SW|Localization]

  • localizes to cell poles in competent cells [Pubmed|16009133]
  • localizes to the DNA entry pole during transformation [Pubmed|22373918]
  • transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery [Pubmed|17630974]

    • Expression and Regulation

    Operons

    genes
    [gene|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK]: activation, [Pubmed|11948146], in [regulon|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|ComK regulon]

    • regulation

  • expressed at the onset of stationary phase [Pubmed|14762004]
    • view in new tab

    genes
    [gene|5BB6CE1D206F2BC278FD2F23CDAAE51CDAFFA2B6|ywpG]-[gene|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]
    description
    [Pubmed|22383849]
    view in new tab

    Biological materials

    Mutant

  • MGNA-A591 (ywpH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/591 NBRP B. subtilis, Japan]
  • BKE36310 (Δ[gene|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE36310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGATTCCTCCTCTCCT, downstream forward: _UP4_TGACACCCCTGCTCCTCCCG
  • BKK36310 (Δ[gene|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK36310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGATTCCTCCTCTCCT, downstream forward: _UP4_TGACACCCCTGCTCCTCCCG

    • Expression vector

  • for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

    • References

    Reviews

  • 22933559

    • Original publications

  • 16009133,17630974,22373918,16549871,14762004,11948146,22383849,22517742,23536821,23779106,25138221,26707201