SubtiBank SubtiBank
metC
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metC

cystathionine beta-lyase
locus
BSU_11880
pI
5.76
mw
42.33 kDa
protein length
390 aa Sequence Blast
gene length
1170 bp Sequence Blast
function
biosynthesis of methionine
product
cystathionine beta-lyase
essential
no
ec
4.4.1.8
synonyms
yjcJ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.10|Biosynthesis/ acquisition of methionine/ S-adenosylmethionine]

    • Gene

    Coordinates
    1,259,606 → 1,260,778

    The protein

    Catalyzed reaction/ biological activity

  • H2O + L,L-cystathionine --> L-homocysteine + NH4+ + pyruvate (according to UniProt)
  • S-substituted L-cysteine + H2O --> thiol + NH4+ + pyruvate (according to UniProt)

    • Protein family

  • trans-sulfuration enzymes family (with [protein|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|MccB] and [protein|04DC792180FDC5E7916F2DBB2EC2C34369202FE0|MetI], according to UniProt)

    • Paralogous protein(s)

  • [protein|314CB4923D15140E72B0ABAFE3E8BE9CA7310E10|MccB], [protein|04DC792180FDC5E7916F2DBB2EC2C34369202FE0|MetI]

    • [SW|Cofactors]

  • PLP (according to UniProt)

    • Structure

  • [PDB|4L0O] (from Helicobacter pylori, 51% identity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|04DC792180FDC5E7916F2DBB2EC2C34369202FE0|metI]-[gene|1C0D38F23D3A2CAA1EB057A92D6F571D0D2AA724|metC]
    description
    [Pubmed|11832514]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11832514], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [regulon|S-box|S-box]: transcription termination/ antitermination, the [SW|S-box] [SW|riboswitch] binds S-adenosylmethionine resulting in termination, in [regulon|S-box|S-box]

    • regulation

  • induced by methionine starvation ([SW|S-box]) [Pubmed|10094622]
  • the [SW|S-box] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]
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    Biological materials

    Mutant

  • MGNA-B299 (yjcJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1298 NBRP B. subtilis, Japan]
  • 1A944 ( ''metC''::''kan''), [Pubmed|11832514], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A944&Search=1A944 BGSC]
  • 1A940 ( ''metC''::''spec''), [Pubmed|11832514], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A940&Search=1A940 BGSC]
  • BKE11880 (Δ[gene|1C0D38F23D3A2CAA1EB057A92D6F571D0D2AA724|metC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGGGTTTCCAGCGTCCAAT, downstream forward: _UP4_TAAAAAAGAAGCCGGGGATA
  • BKK11880 (Δ[gene|1C0D38F23D3A2CAA1EB057A92D6F571D0D2AA724|metC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTGGGTTTCCAGCGTCCAAT, downstream forward: _UP4_TAAAAAAGAAGCCGGGGATA

    • References

  • 19258532,11832514,18039762,12107147