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kbl

2-amino-3-ketobutyrate CoA ligase

locus

BSU_17000

6.25

43.12 kDa

protein length

392 aa Sequence Blast

gene length

1179 bp Sequence Blast

function

threonine utilization

product

2-amino-3-ketobutyrate CoA ligase

essential

2.3.1.29

synonyms

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.8|Utilization of threonine/ glycine]

Gene

Coordinates

1,771,517 1,772,695

The protein

Catalyzed reaction/ biological activity

H⁺ + L-alanine + pimeloyl-[ACP] --> 8-amino-7-oxononanoate + CO₂ + holo-[ACP] (according to UniProt)

Protein family

[SW|class-II pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

Paralogous protein(s)

[protein|8B6A3E86A88928526D94FDA94B9C81ABF6E9ACFB|BioF]

[SW|Cofactors]

PLP (according to UniProt)

Structure

[PDB|1FC4] (from ''Escherichia coli'', 40% identity, 60% similarity) [Pubmed|11318637]

Expression and Regulation

Operons

genes

[gene|8E64B62F15B92A2AACD68B913909100AE276D114|tdh]-[gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]

description

[Pubmed|22383849]

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Biological materials

Mutant

BKE17000 ([gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCCCTTTATG, downstream forward: _UP4_TAACAAGAGGAAGAGTTTAC

BKK17000 ([gene|1BC05699DEB3A16944FB8305F22976AED75805E8|kbl]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17000 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTTTATCCCCCTTTATG, downstream forward: _UP4_TAACAAGAGGAAGAGTTTAC

References

11318637,22960854