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ispF
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ispF

2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, 5th step in the MEP pathway of isoprenoid biosynthesis
locus
BSU_00910
pI
5.40
mw
16.98 kDa
protein length
158 aa Sequence Blast
gene length
477 bp Sequence Blast
function
MEP pathway of isoprenoid biosynthesis
product
2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase
essential
yes
ec
4.6.1.12
synonyms
yacN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    110,480 110,956

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • 4-CDP-2-C-methyl-D-erythritol 2-phosphate --> 2-C-methyl-D-erythritol 2,4-cyclic diphosphate + CMP (according to UniProt)

    • Protein family

  • IspF family (single member, according to UniProt)

    • Structure

  • [PDB|5IWX] (native) [Pubmed|29335298]
  • [PDB|5IWY] (complex with CMP) [Pubmed|29335298]

    • Expression and Regulation

    Operons

    genes
    [gene|179FDEA51A060DF32A3EA06A796793DE27B600FF|yacL]-[gene|CF9633F769263C6D16EE778F6BD2ECF1995F512A|ispD]-[gene|1A6793A3A5AC568C67873C8708E9F581F42AB9BA|ispF]
    description
    [Pubmed|10482513]

    sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|17434969], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]

    • regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]
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    Biological materials

    Mutant

  • MGNA-B882 (yacN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1881 NBRP B. subtilis, Japan]

    • References

  • 15805528,10482513,12270818,17458547,23840410,15502301,29335298