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parE
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parE

subunit of DNA topoisomerase IV
locus
BSU_18090
pI
6.17
mw
72.83 kDa
protein length
655 aa Sequence Blast
gene length
1968 bp Sequence Blast
function
chromosome segregation and compaction
product
subunit of DNA topoisomerase IV
essential
yes
ec
5.99.1.-
synonyms
grlB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • Gene

    Coordinates
    1,933,477 1,935,444

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • upon overexpression, topoisomerase IV ([protein|1901C52DCB24B3D53150F6926C14907AC1B75293|ParE]-[protein|9075DD0CC0B8554F0379732453665F43C70EFC26|ParC]) can take over the functions of the type 1A topoisomerases [protein|41199C76DF8CA804B7B8878D61228B9542A96AE4|TopA] and [protein|7D5327BD1DD5FD5F90B2C849589923AA3D8B20EF|TopB] [pubmed|30957856]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA (according to UniProt)

    • Protein family

  • type II topoisomerase [protein|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|GyrB] family (together with [protein|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|GyrB]) (according to UniProt)

    • Paralogous protein(s)

  • [protein|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|GyrB]

    • [SW|Domains]

  • [SW|TOPRIM domain] (aa 423-537) [Pubmed|9722641]

    • Structure

  • [PDB|4I3H] (from ''Streptococcus pneumoniae'', 70% identity) [Pubmed|23965305]

    • [SW|Localization]

  • Nucleoid (Homogeneous) [Pubmed|16479537]
  • uniformly distributed throughout the cell [Pubmed|9539793]

    • Expression and Regulation

    Operons

    genes
    [gene|1901C52DCB24B3D53150F6926C14907AC1B75293|parE]-[gene|9075DD0CC0B8554F0379732453665F43C70EFC26|parC]
    description
    [Pubmed|16267290]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|30957856], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

    • regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [Pubmed|16267290]

    • additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''parE'' [Pubmed|27120414]
    • view in new tab

    Biological materials

    lacZ fusion

  • GP2550 ([gene|1901C52DCB24B3D53150F6926C14907AC1B75293|parE]::lacZ cat, based on [SW|pAC5]), available in [SW|Jörg Stülke]'s lab [pubmed|30957856]

    • References

    Reviews

  • 16936698

    • Original publications

  • 16272394,9539793,16267290,16479537,23965305,27120414,9722641,12767818,10713132,9334322,8940171,7590259,10944214,1330320,12857958,11090135,8104339,8227000,30222737,30957856